|
Plasmid
Name: pAdeno.Rdmyc Stock #: 1
Strain:DH5α Description: full coding region of
rat desmin cDNA subcloned into the modified pAd.CMV vector. Used for making
adenovirus Created by: Klymkowsky-Oct 95 |
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Plasmid
Name: pAdeno.mycEp-CADTAIL.Myc Stock
#: 2
Strain:DH5α Description: myc Xenopus
EP-Cadherin Tail domain.myc inserted into pAdeno for making adenvirus. pAdCMV. Defective Created by: Klymkowsky-Oct 95-Box 2 |
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Plasmid
Name: pAdeno.mycDGlTAIL.Myc Stock #: 3
Strain:DH5α Description: the bovine desmoglein
tail domain with tags at each end, inserted into the pAdeno vector Created by: Klymkowsky-Oct 95-Box 1 |
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Plasmid Name: pT7*RDM-1 Stock #: 4 Strain:DH5α Description: contains a mutated
form of the rat desmin cDNA in which there is a predicated dominant mutation
from Arg to Cys at the beginning of the rod region. This construct was made
using the PCR overlap; it will be sequenced to confirm the mutation. Created by: Klymkowsky-Nov 95-Box 1 |
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Plasmid Name: pAdeno.Dsc1b.myc Stock #: 5 Strain:DH5α Description: contains a mutated
form of the rat desmin cDNA in which there is a predicated dominant mutation
from Arg to Cys at the beginning of the rod region. This construct was made
using the PCR overlap; it will be sequenced to confirm the mutation. Created by: Klymkowsky-Nov 95-Box 1 |
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Plasmid
Name: pAd.CMV.cat.D
Stock #: 6 Strain:DH5α Description: original adenovirus
plasmid for construction of replication defective virus Created by: Kass-Eisler and
Leinward |
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Plasmid
Name: pAdeno.RDM-3
Stock #: 7 Strain:DH5α Description: Dominant mutation of
rat desmin (myc) Met 262 changed to Arg 262 Created by: Damon Thomas |
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Plasmid Name: pAdeno. Dsc1b.myc
Stock #: 8 Strain:DH5α Description: a recessive mutation in the rat desmin
sequence, apparent generated during PCR amplification. Note: according to Bob
Evans it does not assemble correctly in IF-free cells but does co-assemble
with vimentin Created by: The great unknowable PCR spirit |
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Plasmid
Name: pAdeno.XDesmin.myc
Stock #: 9 Strain:DH5α Description: the
full length xenopus desmin cDNA with myc tag. Derived from plasmids used in
the Cary & Klymowsky papers. I think that this plasmid has only a single
Bam Hl site; it is derived from a second plasmid with a second Bam HI site in
the promoter. We are currently testing whether the removal of this site
effects expression. Created by: M.W. Klymkowsky Checked: March 2010 |
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Plasmid
Name:pT7*mycXPg
Stock #: 10 Strain:DH5α Description: encodes
an N-Terminally tagged, full length version of Xenopus Plakoglobin. Created by: M.W. Klymkowsky Checked: March 2010 |
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Plasmid Name:pT7*m Head+CR PKG
Stock #: 11 Strain:DH5α Description: PCR
derived mutation of Xenopus Plakoglobin in which tail domain is removed. Created by: Dana Dodd-1994 Checked: March 2010 |
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Plasmid Name:pT7*mCR PKG
Stock #: 12 Strain:DH5α Description: PCR
derived mutation of Xenopus Plakoglobin in which head and domains have been
removed. Created by: Dana Dodd- Klymkowsky lab Checked: March 2010 |
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Plasmid
Name:pT7*mCR+Tail PKG
Stock #: 13 Strain:DH5α Description: PCR
derived mutation of Xenopus Plakoglobin in which head domain is removed. Created by: Dana Dodd- Klymkowsky lab Checked: March 2010 |
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Plasmid
Name:pSK.GFP
Stock #: 14 Strain:DH5α Description: plasmid containing coding sequence
for Fluorescent green protein. –original protein (not mutated) Created by: ? Checked: March 2010 |
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Plasmid
Name:pT7*mycDgITail
Stock #: 15 Strain:DH5α Description: human desmoglobin-1 tail domain with a
N-terminal myc tag Created by: M.W. Klymkowsky Checked: March 2010 |
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Plasmid
Name:pT7*mycDgITail
(untagged)
Stock #: 16 Strain:DH5α Description: an untagged form of the
desmoglobin tail domain Created by: Alla Karnovsky-Klymkowsky Lab Checked: March 2010 |
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Plasmid
Name:pT7*mycDsc1b.Tail.myc
Stock #: 17 Strain:DH5α Description: the 1b tail domain of bovine desmocollin 1b
with a C-terminal myc-tag Created by: Jose Nieves Checked: March 2010 |
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Plasmid
Name:pT7*mycDsc1a.Tail.myc
Stock #: 18 Strain:DH5α Description: the 1a tail domain of bovine
desmocollin 1 with a C-terminal myc-tag Created by: Alla Karnovsky-Klymkowsky Lab Checked: March 2010 |
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Plasmid
Name:pT7*mycCadtail Stock #: 19 Strain:DH5α Description: the cytoplasmic tail domain of Xenopus EP-©
cadherin with an N-terminal myc-tag Created by: M.W. Klymkowsky Checked: March 2010 |
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Plasmid
Name:pAd*CMV.cat.D Stock #: 20 Strain:DH5α Description: a version of the original
pAd.CMV.cat.D plasmid with an upstream (unique) Nde I site removed. Tested by
Alison Kass-Eisler and found to be active transcriptionally. Created by: M.W. Klymkowsky Checked: March 2010 |
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Plasmid
Name:pSP6.mycXmango
Stock #: 21 Strain:DH5α
Description: Plasmid contains a myc-taggged
form of the Xenopus mago nashi cDNA for synthesis and injection of RNA. Created by: Phil Newmark-Boswell lab Checked: March 2010 |
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Plasmid
Name:pT7*Dsc3a.Tail.myc Stock #: 22 Strain:DH5α
Description: the (human) desmocollin -3a tail
domain with a C-terminal myc tag Created by: Alla Karnovsky-Klymkowsky Lab Checked: March 2010 |
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Plasmid
Name:
pT7*mDglTail.GFP (old)
Stock #: 23 Strain:DH5α
Description: a chimera of the GFP polypeptide
linked to the C-terminus of the mycDgTail region. In this plasmid the 3ÕUTR
has been deleted! Created by: Jurgen Schmeinsky-Klymkowsky lab Checked: March 2010 |
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Plasmid
Name:pT7*mDglTail.GFP
(new) Stock #: 24 Strain:DH5α
Description: GFP region contains mutation
Ser65-Thr65 ; said to make the fluorescence
brighter, faster and better in every way. Note: This plasmid also does not
have a 3ÕUTR. Created by: M.W. Klymkowsky Checked: March 2010 |
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Plasmid
Name:pSP6.Hum.GSK3B
Stock #: 25 Strain:DH5α
Description: wild type of GSK3b in an SP6
transcription plasmid. Supplied by HE et all (Varmus & Dawid) Created by: HE - (Varmus & Dawid) Checked: March 2010 |
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Plasmid
Name:pSP6.Hum.GSK3B
(dominant) Stock #: 26 Strain:DH5α
Description: dominant mutant form of GSK3b in
an SP6 transcription plasmid. Supplied by HE et all (Varmus & Dawid) Created by: HE - (Varmus & Dawid) Checked: March 2010 |
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Plasmid
Name:pHPGCa2.1 Stock #: 27 Strain:DH5α
Description: full length cDNA clone of Human
Plakoglobin (from Werner Franke) in the pBluescript KS+plasmid. Insert length
3.21 kbp. Accession number M23410—confirmed by EcoRI digest Created by: Michaela Goldschimidt-Franke lab Checked: March 2010 |
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Plasmid
Name:pHPGCa2.1 Stock #: 28 Strain:DH5α
Description: a full length (somewhat rearranged
at the 5Õ end) cDNA of Rat desmin. Created by: Guido Swart Checked: March 2010 |
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Plasmid
Name:pCS2-mt-Mouse
Axin Stock #: 29 Strain:DH5α
Description: this is the orginal RDM-1 mutation
that I planned. It contains a two amino acid change of Asp116.Arg117 to
Glu116.Cys117. Mutated version contains a new Bsm1 site in the region of the
mutation. Created by: M.W. Klymkowsky Checked: March 2010 |
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Plasmid
Name:pACTII Stock #: 30 Strain:DH5α
Description: the plasmid (from Steve Elledge)
that normally contains the cDNA library cloned downstream of the RNA
ploymerse activator domain of Gal4 Created by: Elledge et al Checked: March 2010 |
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Plasmid
Name:pACTII-X/N Stock #: 31 Strain:DH5α
Description: A modified version of pACTII in
which an Xho I and Not I linker has been inserted in order to accept the
Xenopus stage 30 cDNA library. Confirmed by sequencing. Created by: Mark Driver- Klymkowsky lab Checked: March 2010 |
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Plasmid
Name:pAS.CYH Stock #: 32 Strain:DH5α
Description: The shuttle vector used in the
yeast two-hybrid system. Contains DNA binding domain of GAL4 with downstream
prey. Created by: Elledge et al Checked: March 2010 |
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Plasmid
Name:pAS.DesminHead.myc
Stock #: 33 Strain:DH5α
Description: the head of xenopus desmin linked
to the DNA-binding domain of GAL4 in the yeast shuttle vector pAS.CYH Created by: M.W. Klymkowsky Checked: March 2010 |
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Plasmid
Name:pAS.Vimhead.myc
Stock #: 34 Strain:DH5α
Description: the head of vitemntin in yeast
two-hybrid plasmid pAS.CYH Created by: M.W. Klymkowsky Checked: March 2010 |
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Plasmid
Name:pAS.Dsc1b.myc Stock #: 35 Strain:DH5α
Description: the tail domain of bovine
desmocollin 1b.myc in the yeast two-hydrid vector Created by: M.W. Klymkowsky Checked: March 2010 |
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Plasmid
Name:pCS2-mt-Mouse
Axin
Stock #: 36 Description: the full length mouse Axin
subcloned into pCS2mt Created by: Li Zeng-Constantini lab Checked: March 2010 |
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Plasmid
Name:pCS2-mt-Mouse
Axin Δ-RGS
Stock #: 37 Strain:DH5α
Description: this is a domiany mutant of mouse
Axin in the pCS2mt plasmid missing the RGS domain Created by: Li Zeng-Constantini lab Checked: March 2010 |
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Plasmid
Name:pAS.mXMago
Stock #: 38 Strain:DH5α Description: this is a myc-tagged form of the
Xenopus mago nashi cDNA(isolated by the Boswell lab)
placed in the pAS.CYH Plasmid. It does not activate LacZ gene expression on
its own. Created by: Adam Rubenstien- Klymkowsky lab Checked: March 2010 |
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Plasmid
Name:pT7*mycXPkg.4+10
Stock #: 39 Strain:DH5α
Description: this plasmid contains arm repeats
4 to 10 of Xen. Plakoglobin subcloned behind the myc tag Created by: Adam Rubenstien- Klymkowsky lab Checked: March 2010 |
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Plasmid
Name:pAdeno.XenDesmin.myc Stock #: 40 Strain:DH5α
Description: a step on the way to rest of the
pAdeno plasmids, has 2 BamHI sites Created by: M.W. Klymkowsky Checked: March 2010 |
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Plasmid
Name: No
Plasmid
Stock #: 41 Strain:GM2163
Description: this is a dam/dcm-strain from NEB
for manipulating plasmids with methylase sensitive restriction sites Created by: NEB Checked: March 2010 |
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Plasmid
Name: No
Plasmid
Stock #: 42 Strain:XL-1 Blue
Description: Bacterial cell strain Created by: mystery Checked: March 2010 |
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Plasmid
Name: No
Plasmid
Stock #: 43 Strain:DH5α Description: Standard cells for transformation Created by: - Checked: March 2010 |
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Plasmid
Name: No
Plasmid
Stock #: 44 Strain:XL2-Blue
Description: stratageneÕs supercompetant cells Created by: stratagene Checked: March 2010 |
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Plasmid
Name: No
Plasmid
Stock #: 45 Strain:JM109
Description: for transforming with bacteria Created by: - Checked: March 2010 |
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Plasmid
Name:pXenopus
Plakoglobin
Stock #: 46 Strain:JM103
Description: original xen. PKG cDNA from
Berdadette Foquet and Werner Franke Created by: Berdadette Foquet and Werner Franke Checked: March 2010 |
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Plasmid
Name:pXenopus
EP ©-Cadherin
Stock #: 47 Strain:JM103
Description: a full length cDNA cloned of
Xenopus C-Cadherin Werner Franke Created by: Barry Gumbiner et al Checked: March 2010 |
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Plasmid
Name:pXT-Xbra
Stock #: 48 Strain:JM103
Description: the xenopus brachyury cDNA in a
plasmid that can be used to make anti-sense probe for in situ hybridization Created by: Unknown Checked: March 2010 |
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Plasmid
Name:pT7*Xvimentin-1.myc Stock #: 49 Strain:JM103 Description: the full coding sequence of
xenopus vimentin-1 with a C-terminal tag. Created by: Leilah Backhus- Klymkowsky lab Checked: March 2010 |
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Plasmid
Name:pSP6.XVimentin-2.myc
Stock #: 50 Strain:JM109 Description: the full coding sequence of
Xenopus vimentin-2 with a C-terminal myc tag in the pSP64T plasmid. Created by: Joseph Dent-Klymkowsky lab Checked: March 2010 |
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Plasmid
Name:pET.XVimentin-1.stop
Stock #: 51 Strain:BL21 DE3 pLysS Description: Bacterial protein synthesis vector
containing vimentin-1 coding sequence with a stop codon (to tag) Created by: Alberto Domingo-Klymkowsky lab Checked: March 2010 |
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Plasmid
Name:pH7-ÒgoosecoidÓ
in Jm 103
Stock
#: 52A Strain:DH5α Description: this is the wild type Xenopus
GSK3b from David Kimelman Created by: David Kimelman Checked: March 2010 |
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Plasmid
Name:pSP6.XGsk3(wild
type)
Stock
#: 52B Strain:DH5α Description: this is the wild type Xenopus
GSK3b from David Kimelman Created by: David Kimelman Checked: March 2010 |
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Plasmid
Name:pSP6.XGsk.Dominant
Mutation
Stock
#: 53 Strain:DH5α Description: a dominant mutation of Xenopus
Gsk3 Created by: David Kimelman Checked: March 2010 |
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Plasmid
Name:pT7*Bov.Desmocollin-1b.full.myc
Stock #: 54 Strain:DH5α Description: this is a full length cDNA of
desmocollin 1b (myc-tag) Created by: Alla Karnovsky-Klymkowsky lab Checked: March 2010 |
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Plasmid
Name:pET.mycSDesminTail
Stock
#: 55 Strain:DH5α Description: this is a plasmid, proportedly
sequenced by RB Cary and correct in the protein Xba I and
EcoRI sites are in the coding region. Created by: RB Cary-Klymkowsky lab Checked: March 2010 |
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Plasmid
Name:pET.mycVimTail
Stock #: 56 Strain:DH5α Description: Xenopus
vimentin-1Õs tail domain. Created by: RB Cary-Klymkowsky lab Checked: March 2010 |
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Plasmid
Name:pT7*mycXPkg.FGP
Stock
#: 57 Strain:DH5α Description: mutated form of the FGP protein in
frame at C-terminus of XPKG Created by: Pam Yenkinson-Klymkowsky lab Checked: March 2010 |
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Plasmid
Name:pSP6.XKl.tag
in Jm103
Stock
#: 58A Strain:XL2
Description: this is the RMD-3 mutation in
which M 262 is changed to an Arg; made by the two-PCR/overlap method. Needs
to be confirmed by sequencing. Created by: M.W. Klymkowsky Checked: March 2010 |
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Plasmid
Name:pT7*RDM-3
Stock
#: 58B Strain:XL2
Description: this is the RMD-3 mutation in
which M 262 is changed to an Arg; made by the two-PCR/overlap method. Needs
to be confirmed by sequencing. Created by: M.W. Klymkowsky Checked: March 2010 |
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Plasmid
Name:pT7*mycDesminTail
Stock
#: 59 Strain:DH5α Description: this is the new DesminTail
construct subcloned into pT7*. It will be checked by TnT reaction and
subcloned into pAS.CYH. Created by: M.W. Klymkowsky Checked: March 2010 |
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Plasmid
Name:pAdeno
RDM-3
Stock
#: 60A Strain:DH5α Description: construct made in the Leinwand
Lab, based on VR1012 plasmid with CMV 1E promoter /enchancer and CMV 1EÕUTR
together with ÒBGH terminalÓ region. Contains the FGP mutation that I made
inserted with Xba I and BamHI sites. Created by: Rob-Leinwand Lab Checked: March 2010 |
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Plasmid
Name:pAdeno
RDM-3
Stock
#: 60B Strain:DH5α Description: construct made in the Leinwand
Lab, based on VR1012 plasmid with CMV 1E promoter /enchancer and CMV 1EÕUTR
together with ÒBGH terminalÓ region. Contains the FGP mutation that I made
inserted with Xba I and BamHI sites. Created by: Rob-Leinwand Lab Checked: March 2010 |
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Plasmid
Name:pT7*Xdesmin
Head.myc
Stock
#: 61 Strain:DH5α Description: This is the desmin head region
recovered from yeast cells by plasmid rescue. To be used in screening of two
hybrid library. Created by: Mark Driver-Klymkowsky lab
Checked: March 2010 |
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Plasmid
Name:pAS.CYH.mycXDesmin
tail
Stock
#: 62 Strain:DH5α Description: this is the newly made Desmin Tail
domain inserted into the pAS.CYH plasmid for two hybrid screening. Insert is
240b Created by: Mark Driver-Klymkowsky lab
Checked: March 2010 |
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Plasmid
Name:pSP6.B
catinen (HA)
Stock #: 63 Strain:DH5α Description: this is the Xenopus b-catenin RNA
synthesis plasmid from Barry Gumbiner the protein is HA tagged. To linearize,
cut with EcoR1 Created by: Gimbiner lab Checked: March 2010 |
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Plasmid
Name:pcDNA3
Stock
#: 64 Strain:DH5α Description: adenovirus promoter/enhancer primer Created by: invitrogen Checked: March 2010 |
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Plasmid
Name:pT7*C-cadherin
anchor –part 1
Stock #: 65 Strain:DH5α Description: this is the first part in the
construction of a membrane-anchored form of plakoglobin-contains signal and
N-terminal domain of Xenopus C-cadherin. Created by: M.W. Klymkowsky Checked: March 2010 |
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Plasmid
Name:pT7*myc
ΔN.XPkg
Stock
#: 66 Strain:DH5α Description: a truncation of XPKG up to the
beginning of the putative GSK site (homology domain 1) Created by: M.W. Klymkowsky-95 Checked: March 2010 |
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Plasmid
Name:pT7*myc
Cadtail.FGP
Stock #: 67 Strain:DH5α Description: this is the Xenopus EP-cadherin
tail plasmid, linked in frame to the mutated form of FGP; is also has normal
UTRs Created by: M.W. Klymkowsky Checked: March 2010 |
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Plasmid
Name:pConnexin
Stock #: 68 Strain:DH5α Description: this is a pSK derived (T3 and T7)
that encodes the transmembrane domains of rat connexin-32 linked in frame to
synaptopysin. Created by: Rudolf Leube-Franke Lab Checked: March 2010 |
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Plasmid
Name:pT7*mycCG.Xpkg
Stock
#: 69 Strain:DH5α Description: deletion of the GSK concensus site
from the head of XpKG Created by: M.W. Klymkowsky Checked: March 2010 |
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Plasmid
Name:pET3a*mycDG81Tail
Stock
#: 70 Strain:BL21(DE3) pLysS Description: this is the tail domain of the
type 1 Keratin DG81A with a N-terminal myc-tag placed in the bacterial
expression vector PET3a Created by: Robert Cary-Klymkowsky lab
Checked: March 2010 |
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Plasmid
Name:pET3a*XVimentin-1.GFP(old) Stock #: 71 Strain:BL21(DE3) Description: this encodes a chimera of xenopus
vimentin and FGP. Has been checked and acts as a dominant mutation in vitro Created by: M.W. Klymkowsky Checked: March 2010 |
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Plasmid
Name: pET.Xdesmin.Stop
Stock
#: 72 Strain:BL21(DE3) Description: this plasmid expresses the full
length Xenopus desmin polypeptide in bacteria Created by: Robert Cary-Klymkowsky lab
Checked: March 2010 |
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Plasmid
Name: pET.Xenopus
XK1 myc Stock #: 73 Strain:BL21(DE3) Description: this plasmid expresses the type 2
xenopus Keratin 8 homolog (with a myc-tah) in bacteria. Created by: Alberto Domingo/Jeff Bachant-Klymkowsky
lab
Checked: March 2010 |
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Plasmid
Name:pET3a.XVimentin-1.Myc
Stock
#: 74 Strain:BL21(DE3) Description: plasmid expresses a myc-tagged
form of Xenopus vimentin-1 Created by: Joe Dent and Robert Cary -Klymkowsky lab
Checked: March 2010 |
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Plasmid
Name:pET.XDesmin.myc
Stock #: 75 Strain:DH5α Description: this plasmid encodes a myc-tagged
form of Xenopus desmin Created by: Robert Cary -Klymkowsky lab
Checked: March 2010 |
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Plasmid
Name:pET.XDesminn.myc.FGP
Stock #: 76 Strain:DH5α Description: Plasmid
expresses xenopus desmin-FGP Chimera
Created by: Pam Yenkinson -Klymkowsky lab
Checked: March 2010 |
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Plasmid
Name:pAdeno.mycCadtail.FGp
Stock #: 77 Strain:DH5α Description: the mutated FGP attached inframe
to the myoCadtail sequence in the adenovirus vector. Created by: Pam Yenkinson -Klymkowsky lab
Checked: March 2010 |
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Plasmid
Name:pAdeno.HPkg.myc
Stock #: 78 Strain:DH5α Description: Full length human plakoglobin
sequence, with a C-Terminal myc tag, in the pAdeno plasmid for eukaryotic
expression/adenovirus construction
Created by: M.W. Klymkowsky Checked: March 2010 |
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Plasmid
Name:pT7*HPkg.myc
Stock
#: 79 Strain:DH5α Description: Full length human plakoglobin
sequence, with a C-Terminal myc tag, functions to induce dorsal mesoderm in
the early Xenopus embryo Created by: M.W. Klymkowsky Checked: March 2010 |
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Plasmid
Name:pT7*Cnx.HPkg.myc
Stock #: 80 Strain:DH5α Description: this is the rat connexin
transmembrane domain inserted at the N-terminus of Human Plakoglobin-contains
an EcoRI site to facilitate subcloning of other N-Terminal myc-tagged cDNAs
as required. Created by: M.W. Klymkowsky-1996 Checked: March 2010 |
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Plasmid
Name:pET
myc-cys-Vim Tail
Stock
#: 81 Strain:DH5α Description: this is the vimentin tail with a
cys-inserted between the myc-tag and tail to dimerize it. Made with an
undergrade whose name now escapes me Created by: M.W. Klymkowsky Checked: March 2010 |
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Plasmid
Name:pAdeno.Rat.Desmin-myc
Stock #: 82 Strain:DH5α Description: wile type rat desmin for virus
production. Created by: M.W. Klymkowsky Checked: March 2010 |
|
Plasmid
Name:pB4inteqrin-Total
Stock #: 83 Strain:DH5α Description: this plasmid contains the entire
coding sequence of human b4 integrin Created by: Vito Quarata Lab Checked: March 2010 |
|
Plasmid
Name:pT7*Cnx.HPkg.FGP
Stock
#: 84 Strain:DH5α Description: this plasmid encodes a connexin
transmembrane domain, human plakoglobin fluorescent green protein (mutated
version) chimeric polypeptide. Created by: M.W. Klymkowsky Checked: March 2010 |
|
Plasmid
Name:pT7*HPkg.FGP
Stock #: 85 Strain:DH5α Description: this plasmid encodes a human
plakoglobin-FGP chimera Created by: M.W. Klymkowsky Checked: March 2010 |
|
Plasmid
Name:pET.XK1.FGP
Stock #: 86 Strain:BL21 (DE3) pLysS Description: this is the keratin-FGP construct
made by Jurgen schmeinsky during his time in the lab, there is no 3Õ BamHI
site—dead end ligation Created by: Jurgen schmeinsky Checked: March 2010 |
|
Plasmid
Name:pAdeno.Rat.Desmin(w.t).FGP Stock #: 87 Strain:DH5α Description: in frame chimera of Rat Desmin.FGP Created
by: M.W. Klymkowsky Checked: March 2010 Note:missing |
|
Plasmid
Name:pCS2-XSox3
Stock
#: 88 Strain:DH5α Description: from A Zorn/ no tag Created
by: M.W. Klymkowsky Checked: March 2010 |
|
Plasmid
Name:pAdeno.RDM-2.myc Stock #: 89 Strain:DH5α Description: this is the RDM-2 mutation,
subcloned into the pAdeno vector. It needs to be sequenced by Kurt to be
confirmed Created
by: Kurt Harbold and M.W.
Klymkowsky Checked: March 2010 |
|
Plasmid
Name:pET.RatDesmin.Wildtype.myc
Stock
#: 90 Strain:DH5α Description: rat desmin.myc subcloned into the
pET plasmid for bacterial expression Created
by: M.W. Klymkowsky Checked: March 2010 |
|
Plasmid
Name:pET.RDM-3
Stock #: 91 Strain:DH5α Description: RDM-3.myc subcloned into the pET
plasmid for bacterial expression Created
by: M.W. Klymkowsky Checked: March 2010 |
|
Plasmid
Name:pET.RDM-4
Stock #: 92 Strain:DH5α Description: RDM-4.myc subcloned into the pET
plasmid for bacterial expression Created
by: M.W. Klymkowsky Checked: March 2010 |
|
Plasmid
Name:pSynaptoHPkg
Stock #: 93 Strain:DH5α Description: this plasmid encodes the
transmembrane domain of synaptophysin, linked to the human plakoglobin Created
by: S.Troyanovsky Checked: March 2010 |
|
Plasmid
Name:pT7Dsc1b-FGP
Stock #: 94 Strain:DH5α Description: this is a Dsc1b-FGP chimera Created
by: Adam Ruberstein Checked: March 2010 |
|
Plasmid
Name:pRecXenopus
Fibronectin
Stock
#: 95 Strain:DH5α Description: plasmid contains xenopus
fibronectin ina bacterial expression plasmid Created
by: Doug Desimone Checked: March 2010 |
|
Plasmid
Name:pT7HPq8Δ-myc
Stock #: 96 Strain:DH5α Description: truncation of the HPkg coding
sequence after the 8th arm repeat Created by: Yanni Yourgouraskis-Klymkowsky lab
Checked: March 2010 |
|
Plasmid
Name:pZyxin Stock #: 97 Strain:DH5α Description: to fill Created
by: mary beckerle
Checked: March 2010 |
|
Plasmid
Name:pT7*CAnchor
(no myc)
Stock
#: 98 Strain:DH5α Description: to fill Created
by: Adam
Ruberstein/Mike Klymkowsky
Checked: March 2010 |
|
Plasmid
Name:pT7*Syn
(no myc)
Stock #: 99 Strain:DH5α Description: to fill Created
by: Adam Ruberstein/Mike
Klymkowsky
Checked: March 2010 |
|
Plasmid
Name:pT7*CadXTM
(no myc)
Stock #: 100 Strain:DH5α Description: this is the entire signal,
extracellular and transmembrane domains of Xenopus C-cadherin in the pT7*
plasmid with no myc sequence, there is a 3ÕNde 1 site for subcloning. Created
by: Mike Klymkowsky
Checked: March 2010 |
|
Plasmid
Name:pT7*CadXTM
(no myc)
Stock #: 101 Strain:JM103 Description: this is the entire signal,
extracellular and transmembrane domains of Xenopus C-cadherin in the pT7*
plasmid with no myc sequence, there is a 3ÕNde 1 site for subcloning. Created
by: Mike Klymkowsky
Checked: March 2010 |
|
Plasmid
Name:pT7*SynHPkg-myc
Stock #: 102 Strain:JM103 Description: this is the N-terminal,4
transmembrane portion of rat synaptpphysin (ala troyanovsk) attached to the
HPKG-myc sequence Created
by: Mike Klymkowsky
Checked: March 2010 |
|
Plasmid
Name:pT7*CadXTM-HPkg-myc
Stock #: 103 Strain:DH5α Description: this is the N-terminal region of
C-cadherin (minus the cytoplasmic tail) attached to the HPKg-myc
sequence. Created
by: Mike Klymkowsky
Checked: March 2010 |
|
Plasmid
Name:pT3XnF7-1
Stock
#: 104A Strain:DH5α Description: contains NLS sequence of Xnf7-1 Created
by: Adam Rudenstein
Checked: March 2010 |
|
Plasmid
Name:pT7*HPkg-NLS.myc
Stock #: 104b Strain:DH5α Description: contains NLS sequence of Xnf7-1 Created
by: Adam Rudenstein
Checked: March 2010 |
|
Plasmid
Name:pT7*HPkg-NES-myc
Stock #: 105 Strain:DH5α Description: contains nuclear exclusion
sequence of zyxin Created
by: Adam Rudenstein/
Mike Klymkowsky
Checked: March 2010 |
|
Plasmid
Name:pT7*mycVimTail-myc
Stock #: 106 Strain:DH5α Description: this is the double tagged tail of
xenopus vimentin tail (Cary et al 1994) Created
by: Robbert Carry
Klymkowsky
Checked: March 2010 |
|
Plasmid
Name:pCsCnx
ΔGXBCatenin
Stock #: 106 Strain:DH5α Description: this is the double tagged tail of
xenopus vimentin tail (Cary et al 1994) Created
by: Robbert
Carry-Klymkowsky
Checked: March 2010 |
|
Plasmid
Name:pCsCnx
ΔGXBCatenin
Stock #: 107 Strain:DH5α Description: None Created
by: Klymkowsky
Checked: March 2010 |
|
Plasmid
Name: None listed
Stock #: 108 Strain:Unknown Description: None Created
by:
Checked: March 2010 |
|
Plasmid
Name: pGoosecoid
Stock #: 109 Strain:DH5α Description: This plasmid is used to make
probes for in situ analysis Created
by: DeRobertis lab
Checked: March 2010 |
|
Plasmid
Name: pT7*Sal-VimentinTail-myc
Stock #: 110 Strain:DH5α Description: under construction -used to facilitate the subcloning of plasmids
into the pCskAct Created
by: Mark Driver Klymkowsky lab Checked: March 2010 |
|
Plasmid
Name: pT7*Cite-VimentinTail-myc
Stock #: 111 Strain:DH5α Description: under construction This is a cap-independent translation enchacer
plasmid Created
by: Mike Klymkowsky
Checked: March 2010 |
|
Plasmid
Name: pT7*Canchor-myc
Stock #: 112 Strain:DH5α Description: This is a plasmid that was
constructed by piecing together parts of C-cadherin..
It has a 3Õ myc-tag. Created
by: Mike Klymkowsky
Checked: March 2010 |
|
Plasmid
Name: pXNF7-1
Stock #: 113 Strain:DH5α Description: this is a plasmid that contains
pXNF7 cDNA with number of mutations such that there is an NLS sequence at the
5Õend. Used to construct the pT7HPkg-NLS-myc plasmid Created
by: Larry Etkin et al Checked: March 2010 |
|
Plasmid
Name: pT7Canchor-HPkg-myc
(second) Stock #: 114 Strain:DH5α Description: this is the original canchor (part
1+2) region, subcloned from pT7canchor-myc into the pT7HPkg-myc plasmid (no
second PCR) Created
by: Mike Klymkowsky Checked: March 2010 |
|
Plasmid
Name: pT7(sal-minus)mycVTmyc
Stock #: 115 Strain:DH5α Description: this plasmid has a site near the
BAMHI site, removed to facilitate subcloning into pCskACT plasmid Created
by: Mike Klymkowsky Checked: March 2010 |
|
Plasmid
Name: pCS2-MT
Stock #: 116 Strain:DH5α Description: this plasmid has a Sal I NDE
I-GFP-myc-BamHI insert (no Xba I site) in the pT7* Sal plasmid—can be
used in making GFP RNA of for subcloning into on the way to pCskACT Created by: David turner Checked: March 2010 |
|
Plasmid
Name: pXotch
Stock #: 117 Strain:DH5α Description: plasmids contain full encoding
sequence of Xenopus Notch Created
by: Clark Coffman Checked: March 2010 |
|
Plasmid
Name: pCS2-XotchpDBD-UGP
Stock #: 118 Strain:DH5α Description: contains c-terminal (putative)
disheveled-binding domain of Xenopus Notch within frame UGP in the CS2
plasmid Created
by: Mike Klymkowsky Checked: March 2010 |
|
Plasmid
Name: pT7*mycΔG13ΔXPkg
Stock #: 119 Strain:DH5α Description: this is a deletion after the 13th
arm repeat of the DG form of Xenopus plakoglobin Created
by: Mike Klymkowsky Checked: March 2010 |
|
Plasmid
Name: pT7*mycΔG10ΔXPkg
Stock #: 120 Strain:DH5α Description: deletion of GSK site, deletion following the 10th arm repeat. Created
by: Mike Klymkowsky Checked: March 2010 |
|
Plasmid
Name: pT7*mycΔG6ΔXPkg
Stock #: 122 Strain:DH5α Description: deletion of GSK site, deletion following the 6th arm repeat. Created
by: Mike Klymkowsky Checked: March 2010 |
|
Plasmid
Name: pT7*mycΔG5ΔXPkg
Stock #: 123 Strain:DH5α Description: deletion of GSK site, deletion following the 5th arm repeat. Created
by: Mike Klymkowsky Checked: March 2010 |
|
Plasmid
Name: pT7*Sal-NotB4
Stock #: 124 Strain:DH5α Description: contains C-terminal domain of
human b4 integrin tail domain Created
by: Mike Mazzini Mike Klymkowsky Checked: March 2010 |
|
Plasmid
Name: pT7cite-mycΔGC8Δ.XPkg-myc Stock #: 125 Strain:DH5α Description: this plasmid contains the
cap-independent translation enhancer upstream of the mycΔGC8Δ.XPkg-myc coding
sequence; needs to be tested for translational efficiency—is corrected
by TnT reaction. Created
by: Mike Klymkowsky Checked: March 2010 |
|
Plasmid
Name: pCS2mtXotch-DBD
(stop)--Check Stock #: 126 Strain:DH5α Description: this plasmid contains the
C-terminus of Xenopus notch in a pT7* plasmid (with 5Õtag-myc). Created
by: Yanni Vourgourakis-Klymkowsky lab Checked: March 2010 |
|
Plasmid
Name: pT7*mycDsmHead
Stock
#: 127 Strain:DH5α Description: a N-Terminally tagged version for
the yeast 2-hybrid system—to be subcloned into pAS.CYH Created
by: Mark Driver-Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pT7*mycDsmHead-myc
Stock
#: 128 Strain:DH5α Description: a second version of the
DsmHead-myc plasmid for subcloning into pAS.CYH Created
by: Mark Driver-Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pCS2mt-Arrowhead-UGP
Stock #: 129 Strain:DH5α Description: Full coding sequence of arrowhead
(for Heilig) in the pCS2 plasmid with 5Õ myc and 3Õ UGP tags Created
by: Mike Klymkowsky Checked: April 2010 |
|
Plasmid
Name: pCS2mt-Arrowhead-UGP
Stock #: 130 Strain:DH5α Description: this is a yeast ÒbaitÓ plasmid
with the myc-tag on the N-terminus of the desmin head polypeptide, between
desminÕs head and the Gal4DNA binding domain. Created
by: Mark Driver-Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pT7*HPkg-C5
Δ-myc
Stock #: 131 Strain:DH5α Description: part of the human PKG deletion
series Created
by: Adam Ruberstein-Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pAS1-HPkg-C5Δ-myc.CYH
Stock
#: 132 Strain:DH5α Description: the C5Δ deletion of human
plakoglobin in the yeast bait-plasmid. Created
by: Adam Ruberstein-Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pT7*-HPkg-C5Δ-myc
Stock #: 133 Strain:DH5α Description: part of the C-terminal human PKG
truncation series Created
by: Adam Ruberstein-Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pT7*-HPkg-C10Δ-
myc
Stock #: 134 Strain:DH5α Description: part of the C-terminal truncation
human PKG Created
by: Adam Ruberstein-Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pT7*mvcHpkg-N2A-SuperGP
Stock
#: 135 Strain:DH5α Description: part of the N-terminal deletion
series of human PKG causes severe adhesion defect when expressed in the early
embryo (appears around gastrulation)
Created
by: Adam Ruberstein-Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pT7*mvcHpkg-N2
Δ-SGP
Stock #: 136 Strain:DH5α Description: part of the N-terminal deletion
series of human PKG Created by: Adam Ruberstein-Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pT7*mvcHpkg-N5Δ-SGP
Stock #: 137 Strain:DH5α Description: part of the N-terminal deletion
series of human PKG Created by: Adam Ruberstein-Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pT7*mvcHpkg-N6Δ-SGP
Stock #: 138 Strain:DH5α Description: part of the N-terminal deletion
series of human PKG causes severe adhesion defect when expressed in the early
embryo (appears around gastrulation)
Created by: Adam Ruberstein-Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pSK.SuperGreenProtein
Stock #: 139 Strain:DH5α Description: plasmid recovered from Clark
Coffman contains S-T mutation in GFP Created by: Mike Klymkowsky and Clark Coffman Checked: April 2010 |
|
Plasmid
Name: pSK.UltraGreen
Protein
Stock #: 140 Strain:DH5α Description: contains S-A mutation; Bgl II site
removed Serves as PCR substrate for subcloning into other plasmids (under
construction) Created by: Mike Klymkowsky Checked: April 2010 |
|
Plasmid
Name: pT7TS-HA-XSox17B
Stock #: 141 Strain:DH5α Description: plasmids encoding HA tagged, full
length XSox17 beta sequence. Linearize with Pst1 to make RNA. Injection of
RNA ventralizes early Xenopus embryo. Created by: Aaron Zorn-Gurdon LAB Checked: April 2010 |
|
Plasmid
Name: pCS2*mt
Stock #: 142 Strain:DH5α Description: BamHI site in the original pCS2-MT
plasmid has been removed Created by: Yanni Vourgourakis-Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pCS2mt-SGP
Stock #: 143 Strain:DH5α Description: super green protein in the
pCS2*-MT plasmid Created by: Mike Klymkowsky Checked: April 2010 |
|
Plasmid
Name: pCS2*mt-UGP Stock #: 144 Strain:DH5α Description: Ultra green protein in the
un-modified pCS2 plasmid Created by: Mike Klymkowsky Checked: April 2010 |
|
Plasmid
Name: pCS2*mt-ΔG8ΔXpkg
Stock #: 145 Strain:DH5α Description: DNA/RNA expression plasmid for
analysis Created by: Yanni Vourgourakis-Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pT7*myc-UGP
Stock
#: 146 Strain:DH5α Description: expression plasmid with 5Õ myc and
3*S-A mutant in GFP Created by: Rachel DeMent-Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pT7*mycHPkg-N7Δ-GFP
Stock
#: 147 Strain:DH5α Description: part of the deletion series for
HPKG Created by: Adam Ruberstein-Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pCS2mt-XPkg-UGP
Stock #: 148 Strain:DH5α Description: full length Xenopus plakoglobin in
the pCS2 plasmid Created by: Chris Dutton-Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pT7*myc
NOTB4 Tail
Stock #: 149 Strain:DH5α Description: the not b4 tail region of b4
integrin (human) for subcloning into pACT II- to check desmin/integrin
interactions Created by: Mike Klymkowsky Checked: April 2010 |
|
Plasmid
Name: pCS2mt-HARM5-8-UGP
Stock #: 150 Strain:DH5α Description: human PKG arm repeats 4-8 in the
pCS2-UGP vector Created by: Mike Klymkowsky Checked: April 2010 |
|
Plasmid
Name: pCS2mt-HARM5-8-6-UGP
Stock
#: 151 Strain:DH5α Description: human PKG arm repeats 4-8 in the
pCS2-UGP vector Created by: Mike Klymkowsky Checked: April 2010 |
|
Plasmid
Name: pHuman
Plakophilin-A1
Stock #: 152 Strain:DH5α Description: Full length cDNA of human
plakophilin 1A from Franke Lab Created by: Franke et al Checked: April 2010 |
|
Plasmid
Name: pBand6
protein
Stock #: 153 Strain:DH5α Description: this is a partial cDNA (not
complete) isolated by Wheelock and Johnson by PCR Created by: Johnson & Wheelock Checked: April 2010 |
|
Plasmid
Name: pT7*mycHPkg-N5Δ-HPg
Stock
#: 154 Strain:DH5α Description: deleted from the N-terminus
through the 5th ARM repeat Created by: Adam Ruberstein Checked: April 2010 |
|
Plasmid
Name: pT7*mycHPkg-N7Δ-HPg
Stock
#: 155 Strain:DH5α Description: deleted from the N-terminus
through the 7th ARM repeat Created by: Adam Ruberstein Checked: April 2010 |
|
Plasmid
Name: pT7*mycHARM
4-10-SGP
Stock #: 156 Strain:DH5α Description: contains arm repeats 4 through 10
of human plakoglobin with an attached SGP Created by: Adam Ruberstein Checked: April 2010 |
|
Plasmid
Name: pCS2-mt-HPlakophilin-UGP
Stock
#: 157 Strain:DH5α Description: this is a full length plakoglobin
with a 5Õ 6X myc tag and a 3ÕUGP sequence Created by: Mike Klymkowsky Checked: April 2010 |
|
Plasmid
Name: pT7*mycHPlpn
Stock #: 158 Strain:DH5α Description: this is the full length human
plakophilin cDNA in the T7 plasmid Created by: Mike Klymkowsky-confirmed by Adam
Ruberstein Checked: April 2010 |
|
Plasmid
Name: pCS2myc-NH3-SGP
Stock
#: 159 Strain:DH5α Description: in this plasmid, myc GFP moiety is
upstream of the desired coding sequence. Unfortunatly, I forgot to include a
stop codon after the Xba I site Created by: Mike Klymkowsky- Adam Ruberstein Checked: April 2010 |
|
Plasmid
Name: pCS2myc-NH3-SGP
Stock
#: 160 Strain:DH5α Description: the complete tail of Xotch with a
5Õ6myc sequence and a 3Õ UGP moiety. Created by: Yanni Vourgourakis Mike Klymkowsky Checked: April 2010 |
|
Plasmid
Name: pCS2mycUGP-XotchDBD
Stock #: 161 Strain:DH5α Description: the putative Òdisheleveled-binding
domainÓ of Xotch with a 5Õ myc-GFP tag Òultra formÓ Created by: Yanni Vourgourakis–Klymkowsky
lab Checked: April 2010 |
|
Plasmid
Name: pCS2mycUGP-Xotch
Total tail
Stock
#: 162 Strain:DH5α Description: this is the complete Xotch
ctyoplastic tail domain with a 5Õmyc GFP (ultra) tag. Created by: Yanni Vourgourakis–Klymkowsky
lab Checked: April 2010 |
|
Plasmid
Name: pCS2mt-HPlpn-GFP
Stock
#: 163 Strain:DH5α Description: plakophilin ready Created by: Jayme Sanders–Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pCS2mt-hAPC-25-GFP
Stock
#: 164 Strain:DH5α Description: APC25 with a 3Õ GFP Created by: Chris Dutton–Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pCS2mycGFP-HAPC25
Stock
#: 165 Strain:DH5α Description: this is the APC25 sequence with a
5Õmyc-SGP attached Created by: Chris Dutton- Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pCS2MT-APC25-SGP
Stock #: 166 Strain:DH5α Description: APC25 with a 3Õ GFP Tag Created by: Chris Dutton-Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pASI.myc.HMPKGN5
Δ.CYH2 Stock #: 167 Strain:DH5α Description: removel of the head of
HPlpn—attached to the N-terminal mycGFP Created by: Yanni Vourgourakis- Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pASI.myc.HMPKGN10
Δ.CYH2 Stock #: 168 Strain:DH5α Description: keratin promoter (epidermal) from
Tom Sargent Created by: Tom Sargent (NIH) Checked: April 2010 |
|
Plasmid
Name: pASI.myc.HMPKGN7
Δ.CYH2
Stock #: 169 Strain:DH5α Description: N-terminal 6x myc-head deleted,
Human Pipn-UGP tail Created by: Yanni Vourgourakis- Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pAS.N
Δ2.PKG
Stock #: 170 Strain:DH5α Description: yeast Created by: adam Checked: April 2010 |
|
Plasmid
Name: pCAR.MT-APC25-SGP
Stock #: 171 Strain:DH5α Description: cardiac promoter with APC (3ÕGFP)
construct in Created by: Chris Dutton-Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pCAR.mycG-APC-25
Stock
#: 172 Strain:DH5α Description: cardiac promoter with APC 5Õ GFP
tagged form of APC25 Created by: Chris Dutton-Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pCAR.mycG-APC-25
Stock #: 173 Strain:DH5α Description: Xenopus cardiac promoter plasmid
(original) Created by: Enrique Amaya (source) Checked: April 2010 |
|
Plasmid
Name: pCAR.mycSGP
Stock #: 174 Strain:DH5α Description: cardiac promoter plasmid with 5Õ
GFP tag Created by: Chris Dutton-Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pCAR-MT-SGP
Stock
#: 175 Strain:DH5α Description: cardiac promoter plasmid with 3Õ
GFP tag—can be used as reporter since GFP includes stop sequence Created by: Chris Dufton-Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: no
information
Stock #: 176 Strain:DH5α |
|
Plasmid
Name: pCS2mt-XTcf3
(stopped)
Stock
#: 177 Strain:DH5α Description: this is a stopped version of the
pCS2mt-XTCF-3 plasmid Created by: Mike Klymkowsky Checked: April 2010 |
|
Plasmid
Name: pT7.XWnt8-green
Stock #: 178 Strain:DH5α Description: this should be the xenopus wnt8
attached in the frame to SGP but the Xba I/ BamHI digests do not look right
(no digest) Created by: Mike Klymkowsky Checked: April 2010 |
|
Plasmid
Name: pCS2mt-Head
of HPpn-1-UGP Stock #: 179 Strain:DH5α Description: head only of PPN-1 localized to
nuclei in A6 Created by: Jayme Sanders/ Yanni Vourgourakis-
Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pCS2mycUGP-ΔC5HPkg Stock #: 180 Strain:DH5α Description: The C5delta construct in pCS2 Created by: Yanni Vourgourakis- Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pCSmt-HPPn-1-UGP
Stock
#: 181 Strain:DH5α Description: Full sequence Created by: Klymkowsky Checked: April 2010 |
|
Plasmid
Name: pGEX4T2
Stock
#: 182 Strain:DH5α Description: this is a gst-fusion plasmid Created by: commercial-obtained by the amazing
JG! Checked: April 2010 |
|
Plasmid
Name: pGEX4T3
Stock
#: 183 Strain:DH5α Description: this is another gst-fusion protein
plasmid Created by: commercial-obtained by the amazing
JG! Checked: April 2010 |
|
Plasmid
Name: pCS2MT-HDesmogleinTail-GFP
Stock #: 184 Strain:DH5α Description: this is the desmoglein tail domain
placed in the pCS2 plasmid Created by: Mike Klymkowsky Checked: April 2010 |
|
Plasmid
Name: pT7*mycRatDesmin
head
Stock #: 185 Strain:DH5α Description: the head of rat desmin, used to
make pAS.mycRDsmHead Created by: Adam Ruberstein-Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pCsCnxHPkg-GFP
Stock #: 186 Strain:DH5α Description: this is Cnx anchored plakoglobin
(human) in the pCS7 plasmid, with a 3ÕGFP group-NO MYC SEQUENCE. Plasmid
contains to opposing T7 promoters and no SP6 Created by: Yanni Vourgourakis- Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pCS2mt-DesminHead-UGP
Stock #: 187 Strain:DH5α Description: the desmin head construction in
the pCS2 plasmid. I do not know if this is Xenopus or rat or whether there is
a 5Õ MT. I would check it before I used it. Created by: Kira Wendorf- Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pUC8-C1-rat
plectin Stock #: 188 Strain:DH5α Description: this is a protein of rat plectin
cDNA Created by: Gerhard Wiche Checked: April 2010 |
|
Plasmid
Name: pAS1.mycRatDesminHead-CYH2 Stock #: 189 Strain:DH5α Description: for two hybrid screening Created by: Adam Ruberstein-Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: p3.8-N-
β-tubulin-CAT
Stock #: 190 Strain:DH5α Description: the Xenopus neural b-tubulin
promoter driving CAT Created by: Pual Krieg (supplied by Angie
Ribera) Checked: April 2010 |
|
Plasmid
Name: pT7*mycPROX-
β4Tail
Stock #: 191 Strain:DH5α Description: this is the last segment of the
human b4 cytoplasmic tail domain. To be subcloned into the pT7*mycScaβ4Tail Created by: Mike Klymkowsky Checked: April 2010 |
|
Plasmid
Name: pCS2mG-Dsc1aTail
Stock #: 192 Strain:DH5α Description: a CS2 plasmid with a 5Õ mGFP
sequence and ready to be tested Created by: Mike Klymkowsky Checked: April 2010 |
|
Plasmid
Name: pCS2mG-Dsc1bTail
Stock #: 193 Strain:DH5α Description: a CS2 mycGFP version of Dsc1bTail Created by: Mike Klymkowsky Checked: April 2010 |
|
Plasmid
Name: pUC81-Δ13
(epidermal promoter) Stock #: 194 Strain:DH5α Description: this should be the xenopus
epidermal promoter region from Tom Sargent Created by: Tom Sargent/ Chris Dutfon Checked: April 2010 |
|
Plasmid
Name: pT7-HA-XTcf-3 Stock
#: 195 Strain:DH5α Description: Molenaar et all-Xenopus
TCF-1—like transcription factor. Created by: Molenaar et al Checked: April 2010 |
|
Plasmid
Name: pT7-HA-ΔN-XT-cf-3
Stock #: 196 Strain:DH5α Description: Molenaar et all-Xenopus
TCF-1—like transcription factor. Created by: Molenaar et al Checked: April 2010 |
|
Plasmid
Name: pCS2mtXSox-17β-GFP Stock
#: 197 Strain:DH5α Description: an N-Terminal myc-tagged,
C-terminal GFP tagged form of XSox 17β Created by: Klymowsky Checked: April 2010 |
|
Plasmid
Name: pCS2mtXSox-17β
Stock
#: 198 Strain:DH5α Description: an N-Terminal myc-GFP tagged form
of XSox 17β Created by: Klymowsky Checked: April 2010 |
|
Plasmid
Name: pCsCnx-N5Δ-HPkg-GFP Stock
#: 199 Strain:DH5α Description: deletion of head and first four
armsof human plakoglobin in the anchored form. Checked in A6 cells and it
works very nicely. This is the pSC7 plasmid Created by: Klymowsky Checked: April 2010 |
|
Plasmid
Name: pCsCnx-N5Δ-HPkg-GFP Stock
#: 200 Strain:DH5α Description: deletion of head and first four
armsof human plakoglobin in the anchored form. Checked in A6 cells and it
works very nicely. This is the pSC7 plasmid Created by: Klymowsky Checked: April 2010 |
|
Plasmid
Name: pCS2mt-
N5Δ-HPkg-GFP Stock #: 201 Strain:DH5α Description: contains the n5delta version of
HPg in the pCS2mt plasmid Created by: Adam Ruberstein-Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pCS2mt-mycGFP-Proxβ4Tail
Stock #: 202 Strain:DH5α Description: this plasmid contains the region
of β4 integrin proximal to the plasma membrane and running up to the
unique Sca I site Created by: Mike Mazzini -Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pCS2mt-mycGFP-Notβ4Tail
Stock #: 203 Strain:DH5α Description: this is the C-Terminal end of the β4
integrin rail domain attached to a myc-GFP disrupts IF organization in
Xenopus cells by binding to plectin apparently Created by: Mike Mazzini -Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name:
pXEX-UGP
Stock
#: 204 Strain:DH5α Description: this is the mt-UGP Version of thee
Xenopus elongation factor promoter plasmid. Original XEX plasmid from Paul
Krieg Created by: Chris Dufton-Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name:
pSK-epidermal promoter
Stock
#: 205 Strain:DH5α Description: this is the Xenopus epidermal
keratin promoter, subcloned into the pSK plasmid for sequencing. Original
plasmid from Tom Sargent Created by: Chris Dufton-Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name:
pCS2mycG-DsgTail
Stock #: 206 Strain:DH5α Description: an N-terminally tagged form of our
original mycDsgTail plasmid Created by: Mike Klymkowsky Checked: April 2010 |
|
Plasmid
Name:
pAdeno.N5Δ.HPg-myc
Stock #: 207 Strain:DH5α Description: version of plakoglobin for
adenovirus production makes appropriate sized polypeptide Created by: Lori Alger Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name:
pLOX-mGFP-Sca-β4Tail
Stock #: 208 Strain:DH5α Description: a plasmid to be tested to make
adenoviruses—not much success with this. Created by: Mike Klymkowsky Checked: April 2010 |
|
Plasmid
Name:
pLOX-mycGFP
Stock
#: 209 Strain:DH5α Description: as in thee case of stock 208 Created by: Mike Klymkowsky Checked: April 2010 |
|
Plasmid
Name: pGEX2
Stock #: 210 Strain:DH5α Description: this is the modified form of the
pGEX plasmid for subcloning into with EcoRI/Xba I Created by: Julie Gelderloos, Jarmel Chen-
Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name:
pCS2mt-HPpn Head-GFP
Stock #: 211 Strain:DH5α Description: should be the ppn head domain Created by:Klymkowsky Checked: April 2010 |
|
Plasmid
Name: No
information
Stock
#: 212 Strain: Unknown Description: Created by: Checked: April 2010 |
|
Plasmid
Name:
pCS2mycProxB1.GFP
Stock #: 213 Strain: Unknown Description: Created by: Checked: April 2010 |
|
Plasmid
Name:
pT7mScaB4FGP
Stock #: 214 Strain: Unknown Description: Created by: Checked: April 2010 |
|
Plasmid
Name:
pEPI-VGP
Stock #: 215 Strain:DH5α Description: this is the epidermal keratin
promoter driving the expression of GFP Created by: Chris Dufton-Klymkowsky Checked: April 2010 |
|
Plasmid
Name:
pXEX-CnxHPg-GFP
Stock #: 216 Strain:DH5α Description: this is the epidermal keratin
promoter driving the expression of GFP Created by: Chris Dufton-Klymkowsky Checked: April 2010 |
|
Plasmid
Name:
pXEX-Cnx N5Δ HPg-GFP
Stock #: 217 Strain:DH5α Description: this is the Xenopus elongation
factor promoter driving cxn-n5deltaHPg-GFP Created by: Chris Dufton-Klymkowsky Checked: April 2010 |
|
Plasmid
Name:
pT7*C-CAD-myc
Stock #: 218 Strain: Unknown Description: Created by: Checked: April 2010 |
|
Plasmid
Name:
pSP36T-SP6 C-CAD
Stock
#: 219 Strain: Unknown Description: Created by: Checked: April 2010 |
|
Plasmid
Name:
pCS2mt-XTcf-3-UGP
Stock
#: 220 Strain:DH5α Description: a 5Õ myc tagged form of the XTcf-3
transcription factor with a 3ÕGFP Created by: Yanni Vourgourakis- Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pSK-HMG
Box
Stock
#: 222 Strain:DH5α Description: the HMG box XTCF3 (I believe) by itself for making probe. Created by: Amy Hopkins Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name:
pCS2mt-N2D-HPg-GFP
Stock
#: 223 Strain:DH5α Description: the N2D version of plakoglobin with a 5Õ mt and a 3Õ
GFP. Created by: Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name: pET-RDM-4
Stock
#: 224 Strain:? Description: the RDM-4 mutation in pET Created by: Klymkowsky lab Checked: April 2010 |
|
Plasmid
Name:
pET-RDmyc
Stock
#: 225 Strain:? Description: Created by: ? Checked: April 2010 |
|
Plasmid
Name: pT7-
β4 TotalTail-GFP
Stock
#: 226 Strain:Jm109 Description: total tail domain of b4 integrin Created by: Mike Mazzi Klymkowsky Checked: April 2010 |
|
Plasmid
Name:
pCS2mt-BFP
Stock
#: 227 Strain:DH5α Description: blue fluorescent form of the pCS2mt-plasmid Created by: Klymkowsky Checked: April 2010 |
|
Plasmid
Name: pCS2mt
Stock #: 228 Strain:DH5α Description: base plasmid Created by: Turner & Rupp Checked: April 2010 |
|
Plasmid
Name:
pCS2mt-X βCatenin
Stock #: 229 Strain:DH5α Description: full length Xenopus bCatenin in the pCS2 plasmid Created by: Gumbiner lab Checked: May 2010 |
|
Plasmid
Name:
pCS2mG-Scaβ4 Stock #: 230 Strain:DH5α Description: N-terminally mycGFP tagged Sca C-terminal fragment of the b4
integrin cytoplasmic tali domain Created by: Mike Mazzi Klymkowsky Checked: May 2010 |
|
Plasmid
Name:
pCAR-mG-Scaβ4
Stock #: 231 Strain:DH5α Description: Scab4 in the cardiac actin promoter plasmid Created by: Mike Mazzi Klymkowsky Checked: May 2010 |
|
Plasmid
Name: pCS2mG-β4
total tail
Stock #: 232 Strain:DH5α Description: the total cytoplasmic tail of b4 integrin in the pCS2mG Created by: Mike Mazzi Klymkowsky Checked: May 2010 |
|
Plasmid
Name: pCS2ΔG-XβCatenin-BFP
Stock #: 233 Strain:DH5α Description: the deltaG form of XbCatenin attached to BFP Created by: Klymkowsky Checked: May 2010 |
|
Plasmid
Name: pCS2mt-BFP
Stock #: 234 Strain:DH5α Description: BFP in pCS2mt-subcloned using EcoRI and Xba I Created by: Klymkowsky Checked: May 2010 |
|
Plasmid
Name:
pCS2mt-ΔG-XPg-BFP
Stock #: 235 Strain:DH5α Description: the blue form of delta G-XPg Created by: Klymkowsky Checked: May 2010 |
|
Plasmid
Name:
pCS2mt- DG-XPg-BFP
Stock
#: 236 Strain:DH5α Description: the same as stock 235 Created by: Klymkowsky Checked: May 2010 |
|
Plasmid
Name:
pXEX-GFP (5Õ)
Stock
#: 237 Strain:DH5α Description: the xenopus elongation factor promoter Created by: Chris Dufton (I think) Checked: May 2010 |
|
Plasmid
Name: pFull
length human LEF-1
Stock
#: 238 Strain:DH5α Description: the orginal human LEF-1 plasmid Created by: from Kathy Jones Checked: May 2010 |
|
Plasmid
Name:
pCS2mG-hLEF-1
Stock
#: 239 Strain:DH5α Description: the N-terminal GFP tagged form of hLEF1 could be
incorrect-check Created by: Checked: May 2010 |
|
Plasmid
Name:
pCS2mt-hLEF-1-G
Stock
#: 240 Strain:DH5α Description: the C-terminal GFP tagged form it is under that this is
correct needs to be checked Created by: Checked: May 2010 |
|
Plasmid
Name:
pCS2mtRatDesminHead-GFP
Stock #: 241 Strain:DH5α Description: the head of rat desmin with a C-terminal GFP Created by: Kira Wendorf-klymkowsky Checked: May 2010 |
|
Plasmid
Name:
pRSETB-Dsc1aTail
Stock #: 242 Strain: Unknown Description: Dsc1a tail was modified to fit into pRSETB Created by: Julie Gelderloos and Jarmei
chen-Klymkowsky lab Checked: May 2010 |
|
Plasmid
Name:
pRSETB Stock #: 243 Strain: Unknown Description: original plasmid Created by: Checked: May 2010 |
|
Plasmid
Name:
pRSETC
Stock
#: 244 Strain: Unknown Description: original plasmid Created by: Checked: May 2010 |
|
Plasmid
Name:
pCS2mt-ΔG-XPg
Stock #: 245 Strain:DH5α Description: the delta G form, no C-Terminal tag Created by: Chris Dufton Checked: May 2010 |
|
Plasmid
Name:
pCS2mtDG-XPg-RT competitor Stock #: 246 Strain:DH5α Description: as small deletion to be used as a competitor in rtPCR
quantitation Created by: Chris Dufton-Klymkowsky lab Checked: May 2010 |
|
Plasmid
Name:
pCS2mtΔGXbCATENIN
Stock
#: 247 Strain:DH5α Description: as small deletion to be used as a competitor in rtPCR
quantitation Created by: Chris Dufton-Klymkowsky lab Checked: May 2010 |
|
Plasmid
Name:
Stock
#: 248 Strain: Unknown Description: Created by: Checked: May 2010 |
|
Plasmid
Name:
pRSETb.Xpg in BL21
Stock #: 249 Strain: Unknown Description: Created by: Checked: May 2010 |
|
Plasmid
Name:
pSETbDsca1aTail
Stock #: 250 Strain:BL21 Description: desmocollin 1a tail domain Created by: Amy Hopkins Checked: May 2010 |
|
Plasmid
Name:
BL21plysS
Stock #: 251 Strain:BL21 Description: for protein purification Created by: Mike Overduin Checked: May 2010 |
|
Plasmid
Name: BB34
pLysS
Stock #: 252 Strain:BB34 Description: for protein purification Created by: Mike Overduin Checked: May 2010 |
|
Plasmid
Name:
pT7*mycLEF-1
Stock #: 253 Strain:DH5α Description: could be incorrect-check before
using Created by: Yanni Vourgourakis- Klymkowsky lab Checked: May 2010 |
|
Plasmid
Name:
pRSETB-1
Stock #: 254 Strain:DH5α Description: missing Xba I site in original
pRSETB plasmid Created by: Jamie Chen- Klymkowsky lab Checked: May 2010 |
|
Plasmid
Name:
pCsCnxHPg-BFP
Stock #: 255 Strain:DH5α Description: anchored-blue plakoglobin Created by: Klymkowsky Checked: May 2010 |
|
Plasmid
Name:
pRSETb.XpgpLysS in BB34
Stock
#: 256 Strain: Unknown Description: Created by: Checked: May 2010 |
|
Plasmid
Name:
pRSETb.Dsc1aTail+pLysS
Stock #: 257 Strain:B834 Description: for protein purification Created by: Amy Hopkins- Klymkowsky lab Checked: May 2010 |
|
Plasmid
Name:
pRSETb-Dsc1aTail+pLysS
Stock
#: 258 Strain:BL21 Cells Description: for protein purification Created by: Amy Hopkins- Klymkowsky lab Checked: May 2010 |
|
Plasmid
Name:
pRSETb-2
Stock
#: 259 Strain:DH5α Description: this has the original Xba I site
removed and an insert added with Òin frameÓ EcoRI and Xba I sites for
subcloning Created by: Jamie Chen- Klymkowsky lab Checked: May 2010 |
|
Plasmid
Name:
pCS2mt-mNeuroD
Stock
#: 260 Strain:DH5α Description: is the mouse NeuroD protein in
pCS2mt Created by: Jackie Lee Checked: May 2010 |
|
Plasmid
Name:
pCS2mtX12a
Stock #: 261 Strain:DH5α Description: an HLH protein Created by: Jackie Lee Checked: May 2010 |
|
Plasmid
Name:
pRSETB*-XPg
Stock
#: 262 Strain:DH5α Description: the full sequence of xenopus plakoglobin
in the bacterial expression plasmid pRSETB* (modified by us) Created by: Jamei Chen Checked: May 2010 |
|
Plasmid
Name:
pCS2mtβCAT-competitor
Stock #: 263 Strain:DH5α Description: donÕt know whats going on here Created by: Chris Dufton-Klymkowsky lab Checked: May 2010 |
|
Plasmid
Name:
pCS2mt-ND166-XTcf3-GFP
Stock #: 264 Strain:DH5α Description: this is the N-terminally deleted
form of XTcf3-does not bind to catenin and screws up embryos when you inject
the RNA (according to Yanni) Created by: Klymkowsky Checked: May 2010 |
|
Plasmid
Name:
pCsCnxΔG-XPg SGP
Stock #: 265 Strain:DH5α Description: one of the two is wrong, be
careful Created by: Klymkowsky Checked: May 2010 |
|
Plasmid
Name:
pCsCnxN2Δ-HPg-stop
Stock #: 266 Strain:DH5α Description: this is the stopped form-needs to
be checked!- size of insert is not right Created by: Klymkowsky lab Checked: May 2010 |
|
Plasmid
Name:
pCsCnxN5Δ-HPg-stop
Stock #: 267 Strain:DH5α Description: this is the stopped form-seems to
be ok Created by: Klymkowsky Checked: May 2010 |
|
Plasmid
Name:
pCS2mtΔG-XPg-stop
Stock #: 268 Strain:DH5α Description: we have a few of these variants
take care to use the right one Created by: Chris Dufton Checked: May 2010 |
|
Plasmid
Name:
pCS2mtΔG-XPg-CΔ10-stop
Stock #: 269 Strain:DH5α Description: the C-terminally deleted form of
the protein Created by: Chris Dufton Checked: May 2010 |
|
Plasmid
Name:
pCS2mG-XC-CadTail
Stock
#: 270 Strain:DH5α Description: the Green form of the C-cadherin
tail domain Created by: Klymkowsky Checked: May 2010 |
|
Plasmid
Name:
pCS2mG-XSlug
Stock #: 271 Strain:DH5α Description: a N-terminal xenopus slug Created by: Klymkowsky Checked: May 2010 |
|
Plasmid
Name:
pRSETb*-XPg +pLysS
Stock #: 272 Strain:DH5α Description: for protein purification Created by: Amy Hopkins Checked: May 2010 |
|
Plasmid
Name:
pCS2mtΔG-XPg-CΔ13-stop
Stock #: 273 Strain:DH5α Description: a C-terminal truncation plasmid Created by: Chris Dufton Checked: May 2010 |
|
Plasmid
Name:
pT7*mycXSlug
Stock
#: 274 Strain:DH5α Description: a simple myc tagged form of slug Created by: Chris Dufton Checked: May 2010 Note: does not appear to be correct plasmid |
|
Plasmid
Name:
pRSETb-mycNotB4
Stock
#: 275 Strain:DH5α Description: contains myc tag (subcloned from
pT7*myc plasmid) Created by: Mike Mazzi Checked: May 2010 |
|
Plasmid
Name:
pRSetb-mycScaB4
Stock #: 276 Strain:DH5α Description: contains myc tag Created by: Mike Mazzi Checked: May 2010 |
|
Plasmid
Name: pRSetb-mycProxB4
Stock
#: 277 Strain:DH5α Description: for protein purification Created by: Mike Mazzinie Checked: May 2010 |
|
Plasmid
Name:
pRSetb-B4-Total tail
Stock
#: 278 Strain:DH5α Description: for protein purification Created by: Mike Mazzinie Checked: May 2010 |
|
Plasmid
Name:
pT7mouseVimDesmin-myc
Stock
#: 279 Strain:DH5α Description: the head of mouse vimentin on the
body of mouse desmin Created by: Klymowsky Checked: May 2010 |
|
Plasmid
Name:
pCS2mG-XCadtail-stop
Stock
#: 280 Strain:DH5α Description: a stopped version of the XCADTAIL
plasmid Created by: Gerry wilde Checked: May 2010 |
|
Plasmid
Name:
pCS2mG-XSlugΔXba
Stock
#: 281 Strain:DH5α Description: a version of slug with a deletion
from the Xba I site to the end Created by: Andy Ryscavage Checked: May 2010 |
|
Plasmid
Name:
pCsCnxN3Δ-HPg-GFP
Stock
#: 282 Strain:DH5α Description: I was told by the NIH boys that
this contains full length CnxHPg as well. Be careful to check Created by: Klymowsky Checked: May 2010 |
|
Plasmid
Name:
pCsCnxN4Δ-HPg-GFP
Stock
#: 283 Strain:DH5α Description: the N4 version of the GFP-Tagged
form Created by: Klymowsky Checked: May 2010 |
|
Plasmid
Name:
pSP64T-myoD-GR
Stock
#: 284 Strain:DH5α Description: the glucocorticoid repressor
domain attached to mycD Created by: from Hazel Sive Checked: May 2010 |
|
Plasmid
Name:
pSP64T-myoD-ER
Stock #: 285 Strain:DH5α Description: the estrogen repressor domain
attached to myoD Created by: from Hazel Sive Checked: May 2010 |
|
Plasmid
Name:
pCsCnxN3Δ-HPg-stop
Stock
#: 286 Strain:DH5α Description: a stopped form of N3D Created by: Klymowsky Checked: May 2010 |
|
Plasmid
Name:
pRSETb*-XPg
Stock #: 287 Strain:Unknown Description: for protein purification-Xpg
cloned from CNX.Xpg.GFP Created by: Amy Checked: May 2010 |
|
Plasmid
Name:
pCsCnxN2Δ-HPg-stop
Stock #: 288 Strain:DH5α Description: the green form Created by: Klymowsky Checked: May 2010 |
|
Plasmid
Name:
pCsCnxN4Δ-HPg-stop
Stock #: 289 Strain:DH5α Description: a stopped version of N4 Created by: Klymowsky Checked: May 2010 |
|
Plasmid
Name: pCS2mt-ΔG-XβCAT-GR
Stock #: 290 Description: the GR domain attached to the
delta G form of Xbcat Created by: Eric Jensen Checked: May 2010 |
|
Plasmid
Name:
pMX51-cDNA XSna
Stock
#: 291 Description: the cDNA for Xenopus Snail (for
insitu) Created by: Michael Sargent Checked: May 2010 |
|
Plasmid
Name:
pMX363-partail XSlug
Stock #: 292 Strain:DH5α Description: EcoRI fragment for Slug specific
probe synthesis Created by: Michael Sargent Checked: May 2010 |
|
Plasmid
Name:
pMx384.13-pSP72XSlug
Stock
#: 293 Strain:DH5α Description: full length cDNA for Slug Created by: Michael Sargent Checked: May 2010 |
|
Plasmid
Name:
pCS2mtΔG-Y656P-XPg
Stock #: 294 Strain:DH5α Description: mutated form of Xenopus
plakoglobin at a likely tyrosine phosporylation site Created by: Chris Dufton Checked: May 2010 |
|
Plasmid
Name:
pCS2mt-XSlug Stock #: 295 Strain:DH5α Description: the C-terminally tagged form Created by: andy ryscavage Checked: May 2010 |
|
Plasmid
Name:
pAC-CMV-pLpA
Stock
#: 296 Strain:DH5α Description: grows very poorly in LB-use
superbroth Created by: Checked: May 2010 |
|
Plasmid
Name:
pDNA3-S37F-BCAT-ER*
Stock #: 297 Strain:DH5α Description: a point mutant, activated of bCAT
attached to a tamoxifen-responsive ER element Created by: NIH boys Checked: May 2010 |
|
Plasmid
Name:
pT7CnxN3Δ-HPg-GFP
Stock #: 298 Strain:DH5α Description: for making RNA Created by: Yanni Vourgourakis- Klymkowsky lab Checked: May 2010 |
|
Plasmid
Name:
pT7CnxN4Δ-HPg-GFP Stock #: 299 Strain:DH5α Description: for making RNA Created by: Yanni Vourgourakis- Klymkowsky lab Checked: May 2010 |
|
Plasmid
Name:
pRSETb*-DsgTail
Stock
#: 300 Strain:DH5α Description: for protein purification Created by: Amy Hopkins Checked: May 2010 |
|
Plasmid
Name:
pCS2mtΔGXbCAT-RT comp. Stock #: 301 Strain:DH5α Description: this is the competitor for bCAT
rtPCR (quantitative) Created by: Chris Dufton Checked: May 2010 |
|
Plasmid
Name:
pCS2mt-Siamois
. Stock #: 302 Strain:DH5α Description: from the gumbiner lab Created by: Chris Dufton Checked: May 2010 |
|
Plasmid
Name:
pCS2-HA-XSox17β
. Stock #: 303 Strain:DH5α Description: transferred to HA-XSox17b sequence
from pT7 (from Aaron Zorn) to pCS2mt deleted out mt sequence Created by: Klymowsky Checked: May 2010 |
|
Plasmid
Name:
pCS2-HA-XSox17β (stopped)
Stock #: 304 Strain:DH5α Description: derived from the
pCS2mt-XSox17b-GFP constructed—stopped using Xba I/Klenow Created by: Klymowsky Checked: May 2010 |
|
Plasmid
Name:
pSK+XSox2
Stock
#: 305 Strain:DH5α Description: contains XSox2 doing region in
bluescripe SK+cut with Xba I and make antisense RNA using T7 but with EcoRV
and make sense RNA using T3 Created by: from the Grainger lab Checked: May 2010 |
|
Plasmid
Name: pCS2mGFP-XTcf-ΔTail
Stock
#: 306 Strain:DH5α Description: Tail less XTcf3-Does not but with
Xba I due to Dam methylation Created by: Yanni Vourgourakis Checked: May 2010 |
|
Plasmid
Name:
pACCMV- β-catenin*
Stock #: 307 Strain:DH5α Description: β-catenin with S37F mutation
cloned into the Kpn I/Sal I sites of pACCMV Created by: Eric Jensen Checked: May 2010 |
|
Plasmid
Name:
pACCMV-β-catenin*-Δtail
Stock
#: 308 Strain:DH5α Description: β-catenin ΔC-Terminal
Tail (with S37F mutation) cloned into the Kpn I/Sal I sites of pACCMV Created by: Eric Jensen Checked: May 2010 |
|
Plasmid
Name:
pGEX-6P-3
Stock
#: 309 Strain:DH5α Description: GST-fusion plasmid from
Pharmacia Created by: Pharmacia Checked: May 2010 |
|
Plasmid
Name:
pCS2mt-PpnHead-stop
Stock
#: 310 Strain:DH5α Description: plakophilin head stopped-remade in
August 98-correct!!! Created by: Mike Klymkowsky Checked: May 2010 |
|
Plasmid
Name:
pCS2mt-XSlug-ΔXba
Stock #: 311 Strain:DH5α Description: myc tagged xenopus slug with the
xba deletion Created by: Andy Ryscavage-Klymkowsky lab Checked: May 2010 |
|
Plasmid
Name:
pGEX6PXSlug
Stock #: 312 Strain:DH5α Description: slug in GST bacterial sythsesis
vector Created by: Andy Ryscavage-Klymkowsky lab Checked: May 2010 |
|
Plasmid
Name:
pCS2mt-ΔN166-XTcf3
Stock
#: 313 Strain:DH5α Description: the stopped version of the DN166
XTcf3 pCS2mt plasmid Created by: MWK Checked: May 2010 |
|
Plasmid
Name:
pCDNA-HA-XTCF3
Stock #: 314 Strain:DH5α Description: an HA-tagged form of XTCF3 in the
pCDNA plasmid Created by: Clevers lab Checked: May 2010 |
|
Plasmid
Name:
pCDNA-HA-ΔN-XTCF3
Stock #: 315 Strain:DH5α Description: an HA-tagged form DN-XTCF3 Created by: Clevers lab Checked: May 2010 |
|
Plasmid
Name:
pGEX6p-XPg-GFP
Stock #: 316 Strain:DH5α Description: the xenopus plakoglobin-GFP coding
sequence in the GST fusion plasmid Created by: Amy Hopkins Checked: May 2010 |
|
Plasmid
Name: pGEX6p-XTCF3
Stock
#: 317 Strain:DH5α Description: the xenopus XTCF3 sequence in the
GST fusion plasmid Created by: Traci Karmazin Checked: May 2010 |
|
Plasmid
Name:
pGEX6p-XPg-stop
Stock
#: 318 Strain:DH5α Description: the xenopus plakoglobin coding
sequence in the GST fusion plasmid Created by: Amy Hopkins/Jarmei Chen Checked: May 2010 |
|
Plasmid
Name:
pMX5.3*cDNA*XSna*sp72
Stock #: 319 Strain:DH5α Description: full length XSnail plasmid 1.8kB
EcoRI fragment T7 sense/SP6 anti sense Created by: Michael Sargent Checked: May 2010 |
|
Plasmid
Name:
pSiamois -01234-LUC
Stock #: 320 Strain:DH5α Description: the Siamoise promoter plasmid
(driving fire fly luciferase) Created by: Kimmelman Lab Checked: May 2010 |
|
Plasmid
Name:
pSiamois (-)LUC
Stock #: 321 Strain:DH5α Description: the siamois promoter with mutated
TCF sites driving luciferse Created by: Kimmelman Lab Checked: May 2010 |
|
Plasmid
Name:
pTOPFLASH
Stock
#: 322 Strain:DH5α Description: three TCF binding sites driving
fire fly luciferase Created by: Korinek et al Checked: May 2010 |
|
Plasmid
Name:
pFOPFLASH
Stock #: 323 Strain:DH5α Description: the mouse SOX10 cDNA Created by: obtain from Grant Barish Checked: May 2010 |
|
Plasmid
Name:
pmouse Sox10
Stock
#: 324 Strain:DH5α Description: the mouse SOX10 cDNA Created by: obtain from Grant Barish Checked: May 2010 |
|
Plasmid
Name:
pmouse Sox10 Dom
Stock
#: 325 Strain:DH5α Description: the mouse SOX10 dominant mutation Created by: obtain from Grant Barish Checked: May 2010 |
|
Plasmid
Name:
pCRS.XCAD11
Stock #: 326 Strain:DH5α Description: contains 1200 bp cadherin-11
sequence for antisense cut with sac I using T7 for sense, cut with Xho and
use T3/ Created by: Doris Wedlich Checked: May 2010 |
|
Plasmid
Name:
pCDNA-myc-XLEF1
Stock
#: 327 Strain:DH5α Description: the full length cDNA of Xenopus
LEF-1 Created by: Miranda Molenaar Checked: May 2010 |
|
Plasmid
Name: pPS1-HA
Stock
#: 328 Strain:DH5α Description: human presenilin-1 with a
C-terminal HA tag Created by: Ken Kosik lab Checked: May 2010 |
|
Plasmid
Name:
pGEX6p-XβCATENIN
Stock #: 329 Strain:DH5α Description: the xenopus b-catenin sequence in
the GST-fusion plasmid Created by: Jarmei Chen-Klymkowsky Checked: May 2010 |
|
Plasmid
Name:
pCS2mt-Desmoglein tail
Stock
#: 330 Strain:DH5α Description: the desmoglein tail form
pT7-DgITail-Subcloned EcoRI/XBA Created by: Chris Dufton-Klymkowsky lab Checked: May 2010 |
|
Plasmid
Name:
pCS2-mt-C10Δ-Human Plakoglobin Stock #: 331 Strain:DH5α Description: C10D deletion of human
plakoglobin, subcloned into pCS2mt Created by: Chris Dufton-Klymkowsky lab Checked: May 2010 |
|
Plasmid
Name: pCDNA6-XSOX17b-V5-His
Stock
#: 332 Strain:DH5α Description: from Grant Barish Created by: Grant Barish-Varmus Lab Checked: May 2010 |
|
Plasmid
Name:pCDNA6-XSOX17β
Δ 119-373-V5His Stock #: 333 Strain:DH5α Description: deletion of tail domain Created by: Grant Barish-Varmus Lab Checked: May 2010 |
|
Plasmid
Name:pCDNA6-XSOX17β
Δ 119-373-V5His Stock #: 334 Strain:DH5α Description: mouse LEF1 in the pCDNA6 plasmid
with C-terminal V5-HIS tag Created by: Grant Barish-Varmus Lab Checked: May 2010 |
|
Plasmid
Name:pCDNA6-human
TCF4-V5-His Stock #: 335 Strain:DH5α Description: human TCF4 in pCDNA with a
C-terminal V5-His tag Created by: Grant Barish-Varmus Lab Checked: May 2010 |
|
Plasmid
Name:pCS2mt-Xenopus
Dishvelled
Stock
#: 336 Strain:DH5α Description: the xenopus disheveled coding
sequence in the pCS2mt plasmid Created by: Sergei Sokal Checked: May 2010 |
|
Plasmid
Name:pCS2mt-mouse
Dishvelled-1
Stock #: 337 Strain:DH5α Description: mouse dishvelled 1 in an expression
vector Created by: from Grant Barish Checked: May 2010 |
|
Plasmid
Name:pSVK -mouse
Dishvelled-2
Stock #: 338 Strain:DH5α Description: mouse dishevelled Created by: from Grant Barish Checked: May 2010 |
|
Plasmid
Name:pSV-mouse
Dishvelled-3
Stock
#: 339 Strain:DH5α Description: mouse disheveled-3 Created by: from Grant Barish Checked: May 2010 |
|
Plasmid
Name:pHAT-EK-10
Stock #: 340 Strain:DH5α Description: histidine-affinity tag-enterokinase
cleavage site plasmid for generating tagged proteins in bacteria Created by: CloneTech Checked: May 2010 |
|
Plasmid
Name:pSV
-mouse Dishvelled-2
Stock #: 341 Strain:DH5α Description: mouse disheveled-2 Created by: from Grant Barish Checked: May 2010 |
|
Plasmid
Name:pCS2mt-Human
plakoglobin C13Δ Stock #: 342 Strain:DH5α Description: mouse disheveled-2 Created by: Chris Dufton- Klymkowsky lab Checked: May 2010 |
|
Plasmid
Name:pCDNA-XSOX3-V5His
Stock
#: 343 Strain:DH5α Description: XSOX3 with a C-Termina V5His tag Created by: Grant Barish-Varmus lab Checked: May 2010 |
|
Plasmid
Name:pCDNA3-S28A-human
plakoglobin
Stock
#: 344 Strain:DH5α Description: mutated form of human plakoglobin Created by: Grant Barish Checked: May 2010 |
|
Plasmid
Name:pRK5-GSK3β-HA
Stock #: 345 Strain:DH5α Description: I think this came from the Varmus
lab Created by: who knows? Checked: May 2010 |
|
Plasmid
Name:pCDNA3-S37A-β-catenin
Stock #: 346 Strain:DH5α Description: stabilized form of human b-catenin Created by: Grant Barish Checked: May 2010 |
|
Plasmid
Name:pH4x2-LUFICERASE
Stock #: 347 Strain:DH5α Description: SOX response elements in from of
luciferse Created by: Aaron Zorn/Grant Barish Checked: May 2010 |
|
Plasmid
Name:pGEM-T-Rat
Rho
Stock
#: 348 Strain:DH5α Description: has subcloned rat rhoB RT-PCR
product Created by: Eric Jensen-Klymkowsky lab Checked: May 2010 |
|
Plasmid
Name:pCD2mt-Xenopus
Dishevelled
Stock
#: 349 Strain:DH5α Description: xenopus disheveled with an
N-terminal myc tag Created by: Sergai Sokoi (duplicate 7) Checked: May 2010 |
|
Plasmid
Name:pXTwin
Luciferase
Stock #: 350 Strain:DH5α Description: Xtwin promoter construct driving
Luciferase Created by: Ken Cho et al. Checked: May 2010 |
|
Plasmid
Name:pRC-RSV
Stock
#: 351 Strain:DH5α Description: Neomyocin-selectable plasmid with
RSV prometer Created by: INVITROGEN Checked: May 2010 |
|
Plasmid
Name:pRC-RSV*
Stock
#: 352 Strain:DH5α Description: Xba site removed Created by: Klymkowsky Checked: May 2010 |
|
Plasmid
Name:pCD2mt-ΔDesmoplakin-GFP
Stock
#: 353 Strain:DH5α Description: dominant negative mutant form of
desmoplakin (human I think) Created by: Cathy Green Checked: May 2010 |
|
Plasmid
Name:pRcRSV-MT-Humanplakoglobin-CΔ13Stock #: 354 Strain:DH5α Description: inserted Hind III/Not I –
low level expression plasmid Created by: Klymkowsky Checked: May 2010 |
|
Plasmid
Name:pCS2-Luciferase
Stock
#: 355 Strain:DH5α Description: cloned using BamHI and Xba I Created by: Fahlm Ghafoori-Klymkowsky lab Checked: May 2010 |
|
Plasmid
Name:pCS2mt-ΔG-X
b-Catenin
Stock #: 356 Strain:10FÕ Description: same as stock 247 in E.coli strain
designed for more toxic proteins grows better than 247 Created by: Amy Hopkins-Klymkowsky lab Checked: May 2010 |
|
Plasmid
Name:pCDNA6A-V5His
Stock #: 357 Strain:DH5α Description: Expression plasmid Created by: INVITROGEN Checked: May 2010 |
|
Plasmid
Name:pCDNA6-GcR-V5-His
Stock #: 358 Strain:DH5α Description: Expression plasmid with subcloned
glucocorticoid repressor domain Created by: Klymkowsky Checked: May 2010 |
|
Plasmid
Name:
pCDNA6-HA2-XTCF3-GcR-V5His Stock #: 359 Strain:DH5α Description: Expression plasmid Created by: Klymkowsky Checked: May 2010 |
|
Plasmid
Name:pCDNA6-mycXenopus
Lef-1-GcR-V5His
Stock
#: 360 Strain:DH5α Description: Xenopus LEF1 in the GcRespressor
plasmid Created by: Klymkowsky Checked: May 2010 |
|
Plasmid
Name:pCS2-XSOXD
Stock #: 361 Strain:DH5α Description: supplied by Sasai Created by: Sasai Checked: May 2010 |
|
Plasmid
Name:pCS2-GFP*
Stock #: 362 Strain:DH5α Description: GFP with myc-tag removed by Cla /I
EcoRI digest and blunt ligation Created by: Eric Jensen Checked: May 2010 |
|
Plasmid
Name:pCS2-Siamois
minus 3Õ UTR
Stock #: 363 Strain:DH5α Description: pCS2Siamois cut with Xba I-SACII.
Filled in with religated digest with Nco I for linearization reaction Created by: Shoran Rose-Klymkowsky lab Checked: May 2010 |
|
Plasmid
Name:pCDNA6-mycSlug-GcR-V5-His Stock #: 364 Strain:DH5α Description: myc tagged with C-terminal
GcRepressor-V5-His tags Created by: Andy Ryscavage-Klymkowsky lab Checked: May 2010 |
|
Plasmid
Name:pCS2mt-COG-2
Stock
#: 365 Strain:DH5α Description: C.elegans COG-2 cloned into pCS2mt
plasmid with EcoRI/Xba I Created by: Chuck Parsons-Klymkowsky lab Checked: May 2010 |
|
Plasmid
Name:pCDNA6A-HaΔN32-XTCF3-GcR-V5
His Stock #: 366 Strain:DH5α Description: C.elegans COG-2 cloned into pCS2mt
plasmid with EcoRI/Xba I Created by: Chuck Parsons-Klymkowsky lab Checked: May 2010 |
|
Plasmid
Name:pGIEF-B5
Stock
#: 367 Strain:DH5α Description: .XEF1a Rnaase protection probe in
pGEM1 backbone protects 88bp fragment cut with Hind III transcripe probe T7 Created by: Paul Kreig Checked: May 2010 |
|
Plasmid
Name:XSOX3-V5H
Stock
#: 368 Strain:DH5α Description: .Subcloned XLEF1 (Cla I/Xba I)
into the pCDNA-HaHa plasmid (replacing XTCF3) Created by: Klymkowsky Checked: May 2010 |
|
Plasmid
Name:pCDNA6HA2-XSOX17β-V5H
Stock
#: 369 Strain: Unknown
Description: Created by: Klymkowsky Checked: May 2010 |
|
Plasmid
Name:pHAT*XβCAT Stock #: 370 Strain:DH5α Description: Created by: Klymkowsky Checked: June 2010 |
|
Plasmid
Name:pCDNA6A-Ha-XTCF3-V5His Stock #: 371 Strain:DH5α Description: Cloned in Hind III-Xho from
pCDNA6-HA-XTCF3GRHis Created by: Jensen Checked: June 2010 |
|
Plasmid
Name:pCDNA6A-HA-ΔN32XTCF3-V5His Stock #: 372 Strain:DH5α Description: Cloned in Hind III-Xho from
pCDNA6-HA-ΔN32XTCF3-GR-V5His Created by: Eric Jensen Checked: June 2010 |
|
Plasmid
Name:pCS2mt-Snail
Stock
#: 373 Strain: Unknown
Description: snail coding region cloned and
Eco/RI XbaI (not correct plasmid) Created by: K.WEST Checked: June 2010 |
|
Plasmid
Name:pCS2mt-Slug Stock #: 374 Strain: Unknown
Description: snail coding region cloned and
Eco/RI XbaI (not correct plasmid) Created by: Alli and Andy Checked: June 2010 |
|
Plasmid
Name:pCS2mt-XTCF3-V5his
Stock #: 375 Strain:DH5α Description: 5Õ ClaI site is Dom sensitive
XTCF3 inserted Cla Xho Created by: Eric Jensen Checked: June 2010 |
|
Plasmid
Name: pCS2-ΔN32XTCF3-V5His
Stock #: 376 Strain:DH5α Description: ΔN32XTCF3 inserted Cla Xho
ClaI site dom sensitive Created by: Eric Jensen Checked: June 2010 |
|
Plasmid
Name:
pCS2-XSOX3V5His
Stock
#: 377 Strain:DH5α Description: XSOX3=> Cla Xho ClaI site d>m sensitivity. Created by: Eric Jensen Checked: June 2010 |
|
Plasmid
Name:
pCS2-XSOX5V5His
Stock #: 378 Strain:DH5α Description: XSOX5 from Grammer=> Cla Xho
ClaI not dom sensitive Created by: Eric Jensen Checked: June 2010 |
|
Plasmid
Name:
pCS2-XSOXDV5His
Stock #: 379 Strain:DH5α Description: XSOXD from Grammer=> Cla Xho
ClaI not dom sensitive Created by: Eric Jensen Checked: June 2010 |
|
Plasmid
Name:
pCS2-XSOX11-V5His
Stock #: 380 Strain:DH5α Description: XSOX11 inserted Cla Xho ClaI is not dom sensitive Created by: Eric Jensen Checked: June 2010 |
|
Plasmid
Name:
pCS2-XSOX3 ΔN-V5His
Stock #: 381 Strain:DH5α Description: deletion of amino acids 1-155 ClaI
is Dom insensitive Created by: Eric Jensen Checked: June 2010 |
|
Plasmid
Name:
pCS2-XSOX3 ΔC-V5His
Stock
#: 382 Strain:DH5α Description: deletion of amino acids 1-156-310
ClaI is Dom sensitive Created by: Eric Jensen Checked: June 2010 |
|
Plasmid
Name:
pNPG152 (Nrp Probe Template) Stock #: 383 Strain: Unknown
Description: Cut Xba for sensitive probe
Pon-neural Marker Created by: Checked: June 2010 |
|
Plasmid
Name:
p24-10 Neural-Specificβtubutin Stock #: 384 Strain: Unknown Description: Cut Xba for sensitive probe
transcribed T3 pan-neural marker Created by: ? Checked: June 2010 |
|
Plasmid
Name:
pSK*Hairy 2a
Stock
#: 385 Strain: Unknown
Description: Cut BamHI transcribed F7 for
antisense probe Created by: Dave Turner Checked: June 2010 |
|
Plasmid
Name:
pGem-XSOX3 Stock #: 386 Strain: Unknown
Description: Probe template Cut Sol I,
transcribed SP6 for antisense Created by: Yanni Checked: June 2010 |
|
Plasmid
Name:
pBluescript-SK-XAG-1
Stock #: 387 Strain: Unknown
Description: Probe template Cement Gland marker
Cut Not I transcribed antisense probe T7 Created by: Hazel Sive Checked: June 2010 |
|
Plasmid
Name:
pCS105XSOX5.2
Stock #: 388 Strain: Unknown
Description: Probe template 3ÕUTR removed from
original pCS105-XSOX5 Cut Sol I, Transcribed antisense probe T3 Created by: Eric Jensen Checked: June 2010 |
|
Plasmid
Name:
pCS105-XSOX11.2
Stock #: 389 Strain: Unknown
Description: Probe template 3ÕUTR removed from
original pCS105-XSOX11 Cut Sol I, Transcribed antisense probe T3 Created by: Eric Jensen Checked: June 2010 |
|
Plasmid
Name:
pBluescript KS*-en2 Stock #: 390 Strain: Unknown
Description: en 2 probe template, Mid/Hind
brain marker Cut Xba, Transcribed T3 for antisense probes Created by: ? Checked: June 2010 |
|
Plasmid
Name:
pGem4-XKrox 20 Stock #: 391 Strain: Unknown
Description: en 2 probe
template, marker for rhombomers 3,5 in hind brain and neural crest adjacent
to Rhomb 5. Cut EcoRI, transcribed probe T7 Cut Xba, Transcribed T3 for antisense probes Created by: David Wilkinson, Not I institute
for medical research london Checked: June 2010 |
|
Plasmid
Name: DH5
comp cells
Stock #: 392 Strain:DH5α Description: Created by: ? Checked: June 2010 |
|
Plasmid
Name: No
entry for this stock
Stock #: 393 Strain: Unknown
Description: Created by: ? Checked: June 2010 |
|
Plasmid
Name:
pGemblue-XWnt-1
Stock #: 394 Strain:DH5α Description: for in situ probe but Xbe,
transcribe Created by: From O.Destree Checked: June 2010 |
|
Plasmid
Name:
pCS2-XSOX9 Full length
Stock
#: 395 Strain:DH5α Description: Cut with Not I Created by: Shana Fawcett Checked: June 2010 |
|
Plasmid
Name:
p-gen-T-easy-X-SOX9
Stock
#: 396 Strain:DH5α Description: Cut with Sal I Created by: Shana Fawcett Checked: June 2010 |
|
Plasmid
Name: Pax3
Probe Template
Stock
#: 397 Strain: Unknown
Description: for in situ probe linearize Blg II
and transcribe SP6 Created by: from anne Band/ Chris Kintner Checked: June 2010 |
|
Plasmid
Name:
pCS2-XSOX3m7-V5His
Stock #: 398 Strain: Unknown Description: HMG-Box point mutant generated by
Chi Zhang Created by: ? Checked: June 2010 |
|
Plasmid
Name:
pCS2-XSOX3m8-V5His
Stock #: 399 Strain: Unknown
Description: Created by: ? Checked: June 2010 |
|
Plasmid
Name:
pCS2-XSOX3m52-V5His
Stock #: 400 Strain: Unknown
Description: Created by: ? Checked: June 2010 |
|
Plasmid
Name:
pCS2-XSOX3m68-V5His
Stock #: 401 Strain: Unknown
Description: Created by: ? Checked: June 2010 |
|
Plasmid
Name:
pSlug-mtGFP
Stock
#: 402 Strain: Unknown
Description: XSlug Beta Promoter cloned
upstream of mtGFP, replacing the CMV promoter of pCS2*mtGFP Created by: Eric Jensen Checked: June 2010 |
|
Plasmid
Name:
pCS2-XSOX2
Stock #: 403 Strain: Unknown
Description: original cDNA cloned for Sasai
includes endogenous SOX2 UTRÕs inserted Eco/Xho Created by: ? Checked: June 2010 |
|
Plasmid
Name:
pCS2-MT-XSOX3ΔC-Vp16
Stock
#: 404 Strain:DH5α Description: Created by: ? Checked: June 2010 |
|
Plasmid
Name: No
information
Stock
#: 405 Description: Created by: ? Checked: June 2010 |
|
Plasmid
Name: No
information
Stock
#: 406 Description: Created by: ? Checked: June 2010 |
|
Plasmid
Name:
pEuR-XSOX3ΔC
Stock
#: 407 Strain:DH5α Description: insert was cloned into EcoRI Created by: ? Checked: June 2010 |
|
Plasmid
Name:
pBEND2-Xur5WT
Stock
#: 408 Strain:DH5α Description: Created by: ? Checked: June 2010 |
|
Plasmid
Name:
pBEND2-Xur5MUT
Stock
#: 409 Strain:DH5α Description: Created by: ? Checked: June 2010 |
|
Plasmid
Name:
pCS2-NeuroD-Vp16
Stock
#: 410 Strain: Unknown
Description: Created by: Made by D Macora (Lee Lab) Checked: June 2010 |
|
Plasmid
Name: No
information
Stock
#: 411 Strain:
Unknown
Description: Created by: ? Checked: June 2010 |
|
Plasmid
Name: No
information
Stock
#: 412 Strain: Unknown
Description: Created by: ? Checked: June 2010 |
|
Plasmid
Name:
pCS2-VP16-GR Stock #: 413 Strain: Unknown
Description: Created by: ? Checked: June 2010 |
|
Plasmid
Name:
pCS2-ER-GR
Stock #: 414 Strain: Unknown
Description: Created by: ? Checked: June 2010 |
|
Plasmid
Name:XKaiso/pCS2+mt
Stock
#: 415 Strain:DH5α Description: Created by: from Pierre McCrea Checked: June 2010 |
|
Plasmid
Name:XKaiso/pCS2+3HA
Stock
#: 416 Strain:DH5α Description: Created by: from Pierre McCrea Checked: June 2010 |
|
Plasmid
Name:XKaiso/pCS2+C-3HA
Stock
#: 417 Strain:DH5α Description: Created by: from Pierre McCrea Checked: June 2010 |
|
Plasmid
Name:Xath/pBlueScript
SK-
Stock
#: 418 Strain:DH5α Description: Created by: from Brain Parr Checked: June 2010 |
|
Plasmid
Name:XDelta/72-X-Delta-1H/R
Stock #: 419 Strain:DH5α Description: Created by: from Brain Parr Checked: June 2010 |
|
Plasmid
Name:XSOX10/pCS2
Stock #: 420 Strain: Unknown
Description: Sense RNA-linearize with Not I
transcribe with SP6 and Antisense RNA- linearize with Cla I transcribe with
T7 Created by: Yiochiro aobi Checked: June 2010 |
|
Plasmid
Name:NF.Proto-cadherin
Stock
#: 421 Strain:DH5α Description: NFPCDelta SalI/Bs for antisense in
situ: linaerize Not I, transcribe T3 Created by: R Bradley Checked: June 2010 |
|
Plasmid
Name:pCS2+FRL-1
Stock
#: 422 Strain:DH5α Description: Sense probe: linearize Not I,
transcribe sp6, Antisense probe: linearize Cla, transcribe T7 Created by: Yabe et al Checked: June 2010 |
|
Plasmid
Name:Xerl
Stock #: 423 Strain:DH5α Description: for RNA: Not I, sp6 for antisense
probe Created by: T.Kinoshita Checked: June 2010 |
|
Plasmid
Name:
pCS2-XSOX7-GFP
Stock #: 424 Strain:DH5α Description: Created by: Tamara Basta Checked: June 2010 |
|
Plasmid
Name:
Xenopus VEGF cDNA pBS SK+ Stock #: 425 Strain:DH5α Description: for antisense: linearize with BamHI/transcribe
T7 polymerase Created by: Kathryn Tonissen Checked: June 2010 |
|
Plasmid
Name:
ESG-pGEX-4T3
Stock
#: 426 Strain:DH5α Description: Created by: Walter Knochel Checked: June 2010 |
|
Plasmid
Name:
ESG-pCS2-FLAG Stock #: 427 Strain:DH5α Description: Created by: Walter Knochel Checked: June 2010 |
|
Plasmid
Name: pSp
Aol
Stock #: 428 Strain:DH5α Description: template for splicing but with
HincII=> RNA synthesis from T7 promoter Created by: JA Checked: June 2010 |
|
Plasmid
Name:
Xbcl-2 FLAG
Stock #: 429 Strain:DH5α Description: mRNA; linearize BamHI; polymerize
T7 Created by: Mallu lab Checked: June 2010 |
|
Plasmid
Name:
OTX2/pBS
Stock
#: 430 Strain: Unknown
Description: sense cut with HindII, T3 and
antisense cut with NotI, T7 Created by: University of Pen. Klein lab Checked: June 2010 |
|
Plasmid
Name:
XMyoDb for insitu probe
exclusively Stock #: 431 Strain:DH5α Description: pbsks+XMyoDb but with Hindui,
transcribe T7 Created by: Ralph Rupp Checked: June 2010 |
|
Plasmid
Name:
pCS2-n-B-gal Stock #: 432 Strain:DH5α Description: cut with NotI, transcripted by
SP6 Created by: University of Pen. Klein lab Checked: June 2010 |
|
Plasmid
Name:
pRR104-XMycD6
Stock
#: 433 Strain: Unknown
Description: for sense RNA, linearizing Asp
718; transcribed SP6 or NotI Created by: Ralph Rupp Checked: June 2010 |
|
Plasmid
Name:
pCS2-myc-Xsnail 2-GFP
Stock #: 434 Strain: Unknown
Description: cloned by Chi, (site
EcoRI/XbaI Created by: ? Checked: June 2010 |
|
Plasmid
Name:
pCS2-myc-Xsnail 2-GFP
Stock
#: 435 Strain: Unknown
Description: cloned by Chi, (site
EcoRI/XbaI Created by: ? Checked: June 2010 |
|
Plasmid
Name: Human
bcl2
Stock #: 436 Strain:DH5α Description: for RNA: linearize StuI;
transcribe T7 Created by: Gantien lab Checked: June 2010 |
|
Plasmid
Name:
pCS3-Mixer
Stock
#: 437 Strain: Unknown
Description: sense RNA cut with Ascl transcribe
with Sp6 Created by: Gantien lab Checked: June 2010 |
|
Plasmid
Name:
pCS3-GnR-Mixer
Stock
#: 438 Strain: Unknown
Description: Created by: Melton lab Checked: June 2010 |
|
Plasmid
Name:
blue-ScriptII-XSox18cDNA
Stock
#: 439 Strain: Unknown
Description: in EcoRI/Xho1 situ Created by: Yoshiki Hiraoka Checked: June 2010 |
|
Plasmid
Name:
XFGF8
Stock #: 440 Strain: Unknown
Description: Lineaization Xba1 (initu probe) T3
for antisense RNA Created by: ? Checked: June 2010 |
|
Plasmid
Name:
VegT-Eng SacII sp6 Stock #: 441 Strain: Unknown
Description: Lineaization Xba1 (initu probe) T3
for antisense RNA Created by: ? Checked: June 2010 |
|
Plasmid
Name:
pCs2-mt-XPygopus
Stock
#: 442 Strain: Unknown
Description: in NcoI/EcoRI Acc.AF521655 Created by: Belenkaya et al. (2002) Dev.
129(17) 40894101 Checked: June 2010 |
|
Plasmid
Name:p(SK-)-Squint
(Fish)
Stock
#: 443 Strain: Unknown
Description: Created by: ? Checked: June 2010 |
|
Plasmid
Name:p(SK-)-Cyclos
(Fish) Stock #: 444 Strain: Unknown
Description: Created by: ? Checked: June 2010 |
|
Plasmid
Name:pCS2-XSOX7ALT-GFP(in
ClaI/XbaI) Stock #:445 Strain:DH5α Description: alternative from of XSOX7=>for
SOX7MO Rescue Created by: Tamara Basta Checked: June 2010 |
|
Plasmid
Name:pCS2-XSOX18ALT-GFP(in
ClaI/XbaI)Stock #:446 Strain:DH5α Description: alternative from of XSOX7=>for
SOX7MO Rescue Created by: Tamara Basta Checked: June 2010 |
|
Plasmid
Name:GBx2a
Stock #: 447 Strain: Unknown
Description: anti-sense BamHI T7 Created by: Shalom GracielaÕs lab Checked: June 2010 |
|
Plasmid
Name:pCS2-XSOX1-V5H Stock #: 448 Strain: Unknown
Description: for sense RNA SACII linearization,
Sp6 transcription Created by: Chi Zhang Checked: June 2010 |
|
Plasmid
Name:pCS2-XSox7ΔC-GFP
Stock #: 449 Description: in ClaI/EcoRI contains HMG box
+20ava Created by: Tamara Basta Checked: June 2010 |
|
Plasmid
Name:pCS2-Sox1-GFP Stock #: 450 Strain:DH5α Description: in ClaI/EcoRI Created by: Tamara Basta Checked: June 2010 |
|
Plasmid
Name:pCS2+MT
(original plasmid)
Stock #: 451 Strain: Unknown
Description: Created by: Harland lab Checked: June 2010 |
|
Plasmid
Name:BCLg
in pBSIISK backbone
Stock #: 452 Strain: Unknown
Description:
GenBanc Acc.No.XM_563 Created by: Thomas Kramps Checked: June 2010 |
|
Plasmid
Name:pTK1-mtBclg
Stock #: 453 Strain:DH5α Description: 6myc emitope from pCS2+mt vector
was cloned in front of pIK1Balg. For Cap RNA linaerize w SacI and aux.w.Sp6 Created by: Tamara Basta Checked: June 2010 |
|
Plasmid
Name:
pCS2-GR-XSox7-GFP
Stock #: 454 Strain:DH5α Description: GR insert was cloned in front of
pCS2-AltXSox7-GFP into BamHI ClaI sites. In this construct Cla is dam
sensitive Created by: Tamara Basta Checked: June 2010 |
|
Plasmid
Name:pCS2-GR-XSox7ΔC-VP16
Stock #: 455 Strain: Unknown
Description: XSox7ΔC(alt) insert cloned
into BamHI/EcoRI site) Created by: Tamara Basta Checked: June 2010 |
|
Plasmid
Name:pCS2-GR-XSox18ΔC-VP16
Stock #: 456 Strain: Unknown
Description: XSox18ΔC insert cloned into
BamHI/EcoRI site) Created by: Tamara Basta Checked: June 2010 |
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Plasmid
Name:pCS2-GR-XSox3ΔC-VP16
Stock #: 457 Strain: Unknown
Description: XSox3ΔC insert was cloned BamHI
EcoRI sites. This clone is frame with Myc with VP16 Created by: Tamara Basta Checked: June 2010 |
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Plasmid
Name:pCS2-GR-Slug-GFP
Stock #: 458 Strain: Unknown
Description: (slug was inserted in pCS2-GR-GFP
with ClaI :: XbaI site ClaI is dam sensitive ) to make injected RNA
linearized by SACII Created by: Chi Zhang Checked: June 2010 |
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Plasmid
Name:pCS2-GR-XSox3-GFP
Stock #: 459 Strain: Unknown
Description: XSox3C insert cloned into pCS2-GR-GFP
with ClaI :: XbaI site to make injected RNA linearized by SacII or NotI transcribe
with Sp6 Created by: Checked: June 2010 |
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Plasmid
Name:pCS2-UTR-XSox18-V5H
Stock #: 460 Strain: Unknown
Description: (5Õ UTR-XSOX18 was inserted into
pCS2-V5H was Cla2::XhoI to make injected RNA linearized with NotI transcribe
with Sp6 Created by: Chi Zhang Checked: June 2010 |
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Plasmid
Name:C-MYC
(295D8)
Stock #: 461 Strain: Unknown
Description: cut and transcribe with for probe
antisense EcrI/T7 Created by: La Bonne lab Checked: June 2010 |
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Plasmid
Name:MyoCardin
Stock #: 462 Strain: Unknown
Description: cut with BamHI Transcribed with
Sp6 for insitu probe Created by: Pan A.Kreig Checked: June 2010 |
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Plasmid
Name:BKBlue
(reported) in pGL3
Stock #: 463 Strain: Unknown
Description: Created by: ? Checked: June 2010 |
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Plasmid
Name:1KBSA-mutant
Stock #: 464 Strain: Unknown
Description: 1KBd 532A/536A (pCDNA) from 1CS Created by: Tamara Basta Checked: June 2010 |
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Plasmid
Name:pCS2-ΔN35XSOX3
Stock #: 465 Strain: Unknown
Description: (ClaI/EcoRI insert (PCR generated)
of ΔN35XSOX3 cloned into pCS2-GFP vector. Insert contains stop codon
(GM2163 cells) Created by: Tamara Basta Checked: June 2010 |
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Plasmid
Name:pCS2-ΔN35XSOX3.GFP
Stock #: 466 Strain: Unknown
Description: (ClaI/EcoRI insert (PCR generated)
of ΔN35XSOX3 cloned into pCS2-GFP vector. Insert have no stop codon
(into GM2163 cells) NOT GREEN Created by: Tamara Basta Checked: June 2010 |
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Plasmid
Name:pSP64T:XrelA
Stock #: 467 Strain: Unknown
Description: cut by EcoRI, transcribed with Sp6 Created by: ? Checked: June 2010 |
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Plasmid
Name:pSP64T:XrelAΔSP
Stock #: 468 Strain: Unknown
Description: cut by EcoRI, transcribe with Sp6 Created by: Hugh Woodland Checked: June 2010 |
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Plasmid
Name:pCS2-XrelA
Stock #: 469 Strain: Unknown
Description: Created by: ? Checked: June 2010 |
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Plasmid
Name:pCS2-GR-XR11-GFP(in
GM2163 cells)Stock #: 470 Strain: GM2163
Description: contains N-terminal GRE domain
Xenopus XR11 (GuyBack #X82461) and C-Termanial GFP NheI/XbaI between XR11 and
GFP Created by: Tamara Basta Checked: June 2010 |
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Plasmid
Name:pCS2-ΔN35XSOX3V5(in
GM2163cells) Stock#:471 Strain: GM2163
Description: Created by: Tamara Basta Checked: June 2010 |
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Plasmid
Name:pCS2-GR-XRelA Stock #: 472 Strain: Unknown
Description: GR was inserted into ClaI site in front of XRalA) Created by: Chi Zhang Checked: June 2010 |
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Plasmid
Name:pU52-eGFP
Stock #: 473 Strain: Unknown
Description: Created by: Jack Parent university of Michigan Checked: June 2010 |
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Plasmid
Name:pCS2-X1KB-V5H
Stock #: 474 Strain: Unknown
Description: (X2KB who inserted into BamH :
Xho2 site) Linearized by NotI transcribed with Sp6 for RNA Created by: Jack Parent university of Michigan Checked: June 2010 |
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Plasmid
Name:pCS2-WnD
(Fly)-V5H
Stock #: 475 Strain: Unknown
Description: (X2KB who inserted into BamH :
Xho2 site) Linearized by NotI transcribed with Sp6 for RNA Created by: Jack Parent university of Michigan Checked: June 2010 |
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Plasmid
Name:5Cl
Stock #: 476 Strain: Unknown
Description: T7/Xho2 gives antisense for insitu
Created by: Smith lab Checked: dateÉ |
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Plasmid
Name:pUS2-GR-eGFP Stock #: 477 Strain: Unknown
Description: GR was inserted into AatII site
constructed by Created by: Chi Zhang Checked: June 2010 |
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Plasmid
Name:pGBk-XSOX7
Stock #: 478 Strain: Unknown
Description: T7/SPEI give anti-sense probe Created by: Chi Zhang Checked: June 2010 |
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Plasmid
Name:pUS2-GR-eGFP Stock #: 479 Description: GR was inserted into AatII site of
pUS2-eGFP. Note: pDNA injection in Xenopus can see green. Created by: Chi Zhang Checked: June 2010 |
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Plasmid
Name:pG3
cytoskeleton Cepidermal
Stock #: 480 Strain: Unknown
Description: anti-sense BamHI/Sp6 Created by: Harland Checked: June 2010 |
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Plasmid
Name:pME-XrelB
Stock #: 481 Strain: Unknown
Description: anti-sense BamHI/Sp6 Created by: Kazuo Marayama University of Tokyo Checked: June 2010 |
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Plasmid
Name:pDSRed
monomer
Stock #: 482 Strain: Unknown
Description: Created by: Clonetech Checked: June 2010 |
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Plasmid Name:pDSRed2
Stock #: 483 Strain: Unknown
Description: Created by: Clonetech Checked: June 2010 |
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Plasmid
Name:pXR11.DsRed
monomer
Stock #: 484 Strain: Unknown
Description: Created by: Clonetech Checked: June 2010 |
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Plasmid
Name:pCS107-XSOX2 Stock #: 485 Strain: Unknown
Description: sense Sp6 Ascl anti-sense T7
HindII Created by: Clonetech Checked: June 2010 |
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Plasmid
Name:pCS107-XSOX3 Stock #: 486 Strain: Unknown
Description: sense Sp6 Ascl anti-sense T7
HindII Created by: Clonetech Checked: June 2010 |
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Plasmid
Name:pUS2-eGTP/Sal- Stock #: 487 Strain: Unknown
Description: Created by: Clonetech Checked: June 2010 |
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Plasmid
Name:pERTM-XR11-DsRedm
Stock #: 488 Strain: Unknown
Description: Created by: Clonetech Checked: June 2010 |
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Plasmid
Name:pUS2
eGFP/SAL- Not- (Nsi/Not-) Stock #: 489 Strain: Unknown
Description: Created by: Clonetech Checked: June 2010 |
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Plasmid
Name:pUS
eGFP/SAL- Not- (Nsi/Not-) Stock #: 490 Strain: Unknown
Description: Created by: ? Checked: June 2010 |
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Plasmid
Name:pUS2-GR-SOX3-GFP
Stock #: 491 Strain: Unknown
Description: Created by: ? Checked: June 2010 |
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Plasmid
Name:pUS2-GR-SOX7-GFP
Stock #: 492 Strain: Unknown
Description: Created by: ? Checked: June 2010 |
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Plasmid
Name: No
information Stock #: 493 Strain: Unknown
Description: Created by: ? Checked: June 2010 |
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Plasmid
Name: pCDNA3
Stock #: 494 Strain: Unknown
Description: in Dh5a no insert Created by: ? Checked: June 2010 |
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Plasmid
Name: SOX1ΔUTR
(CS108)
Stock #: 495 Strain: Unknown
Description: Created by: Tim grammer Checked: June 2010 |
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Plasmid
Name: Squint
in SK
Stock #: 496 Strain: Unknown
Description: Sense Asp 718/13, antisense
Zcor1/T7 Created by: Chris Wright Checked: June 2010 |
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Plasmid
Name: pCDNA-GR Stock #: 497 Strain: Unknown
Description: Created by: ? Checked: June 2010 |
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Plasmid
Name: pCDNA-GFP
Stock #: 498 Strain: Unknown
Description: Created by: ? Checked: June 2010 |
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Plasmid
Name: pCDNA-GRGFP Stock #: 499 Strain: Unknown
Description: Created by: ? Checked: June 2010 |
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Plasmid
Name: pCDNA-GR-SOX3-GFP
Stock #: 500 Strain: Unknown
Description: Created by: ? Checked: June 2010 |
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Plasmid
Name: pCDNA-GR-SOX7-GFP
Stock #: 501 Strain: Unknown
Description: Created by: ? Checked: June 2010 |
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Plasmid
Name: Xpat
Stock #: 502 Strain: Unknown
Description: AS cut Eco R1, transcribe with T7,
Sense: cut XhoI, transcribe T3 Created by: Woodland lab Checked: June 2010 |
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Plasmid
Name: pCS2-Oct
25 SP6/Not!
Stock #: 503 Strain: Unknown
Description: Created by: Dr. Ying Lao Checked: June 2010 |
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Plasmid
Name: pCS2-Oct
60 SP6/Not!
Stock #: 504 Strain: Unknown
Description: Created by: Dr. Ying Lao Checked: June 2010 |
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Plasmid
Name: pCS2-Oct
91 SP6/Not!
Stock #: 505 Strain: Unknown
Description: Created by: Dr. Ying Lao Checked: June 2010 |
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Plasmid
Name: pCS2-SOX3-GFP
stock #: 506 Strain: Unknown
Description: 5ÕCla1/3ÕXba1 site. VERY GREEN Created by: Chi Zhang Checked: June 2010 |
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Plasmid
Name: pCS2-
ΔN35XSOX3-GFP
Stock #: 507 Strain: Unknown
Description: 5ÕCla1/3ÕXba1 site. VERY GREEN Created by: Chi Zhang Checked: June 2010 |
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Plasmid
Name: pIRES-BASE
PLASMID
Stock #: 508 Strain: Unknown
Description: Created by: ? Checked: June 2010 |
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Plasmid
Name: pCS2-Xolloid Stock #: 509 Strain: Unknown
Description: Not1/Sp6 some RNA Created by: ? Checked: June 2010 |
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Plasmid
Name: pCS2-Cerberus
Stock #: 510 Strain: Unknown
Description: EcoR1/T7 antisense RNA Created by: ? Checked: June 2010 |
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Plasmid
Name: pIRES2::GFP
KanR
Stock #: 511 Strain: Unknown
Description: Created by: Arthur Guiter Houtmen Checked: June 2010 |
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Plasmid
Name: pIRES2.GFPSOX7::GFP
KanR Stock #: 512 Strain: Unknown
Description: Created by: MK Checked: June 2010 |
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Plasmid
Name: pIRES2::GFP
AmpR
Stock #: 513 Strain: Unknown
Description: Created by: MK Checked: June 2010 |